摘要
Triptolide was given orally to adult male Sprague-Dawley rat sat a dosage of 75 μg/kg for 35 days.After 28 days of treatment,the result of mating tests showed that all the drug treated rats were infertile.At the end of drug treatment,the density of caudal spermatozoa and the weight of cpididymis were reduced significantly.All the spermatozoa were immobile.There was no detectable damage of spermatogenesis and epididymal epithelia in triptolide treated rats under microscopical examination.However,modcrate and severe damage of spermatozoa were seen in the corpus and caudal epididymis.The content of cytosolic and nuclear dihydrotestosterone (DHT) receptors in the caput and caudal epididymides was increased but insignificantly as compared with that of the controls.However, the content of DHT receptor in the cytosal of the ventral prostate was elevated very,significantly (P< 0.01).This result suggests that one of the sitcs of action of triptolide might be the epididymis.
雄性 Sprague-Dawley 大鼠口服雷公藤内酯醇(75μg/kg/d)共35天。服药28天后,交配实验表明,所有给药大鼠均已产生抗生育作用。给药35天后,给药组动物体重、睾丸、精囊、腹前列腺及垂体前叶的重量,血清受睾丸间质液中的睾酮浓度;垂体促间质细胞激素(LH)、促卵泡激素(FSH)浓度以及睾丸内 FSH 受体的浓度与对照组相比均无改变;附睾中精子密度及附睾重量明显降低、精子均不活动。在光镜和相差显微镜下观察给药大鼠睾丸生精细胞及附睾上皮细胞没有损伤。然而,在附睾头、尾管腔中的精子分别呈现中度和严重的损伤。如精子头尾断裂,轴丝膨大。给药大鼠附睾头、尾的胞浆及胞核,双氢睾酮受体(DHT)的含量增高,但无显著的统计学意义。腹前列腺胞核受体也无显著差异。而腹前列腺胞浆 DHT 受体与对照组相比有显著升高(P<0.01)。实验结果提示雷公藤内酯醇的抗生育作用部位之一在附睾。
