齐墩果酸对免疫复合物型变态反应的影响

Effects of Oleanolic Acid on the Immune Complex Allergic Reaction and Inflammation

致敏前后灌胃给药(d1～d5,d11～d17)齐墩果酸(OA)对 Arthus 反应无明显影响攻击前48,24,1小时给药则有显著抑制作用,对可逆性被动 Arthus 反应有明显抑制作用,且显著抑制白细胞游走反应。OA 能显著稳定红细胞膜,抑制制霉菌素引起的足肿胀减少其炎症渗出物中酸性磷酸酶含量。OA 明显抑制 PGE、组织胺、LTB_4、激肽的合成或释施 PGE_2、组织胺、5-HT 和激肽的致炎作用,明显减少酒精中毒小鼠肝组织中丙二醛(MDA)含量提高小鼠肝组织中过氧化氢酶(CAT)活性,但对大鼠血清中超氧化物歧化酶(SOD)活性无明显影响。对 Arthus 反应大鼠血清中免疫复合物含量,巨噬细胞清除免疫复合物,单核-巨噬细胞系统吞噬功能及对总补体活性均无明显影响。

When oleanolic acid (OA) was administered ig before and after sensitization on d 1 to d 5 and d 11 to d 17,it had no apparent effect on Arthus reaction.When it was administered at 48,24 and 1 h before challenge,however,Arthus reaction was significantly inhibited.OA showed markedly suppressive effects on reversible passive Arthus reaction and leukocyte migratory response.It could significantly stabilize erythrocyte membrane,inhibit the swelling of the rat's hind paw induced by in- jecting mycostatin,reduce the acid phosphatase content in the inflammatory exudate,suppress the syn- thesis or release of PGE,histamine,LTB4 and kinin,and the phlogistic action of PGE_2,histamine,5- HT and kinin.In addition,it could decrease the content of malonyldialdehyde (MDA) in hepatic tissue of alcohol-intoxicated mice,and increase the activity of catalase (CAT) in hepatic tissue of mice.OA had no apparent effect on the activity of super-oxide dismutase (SOD) in rat serum,on the content of immune complex in serum of rat with Arthus reaction,on the phagocytosis of monocytc-macrophage system,on the clearance of enzyme-containing immune complex by macrophage,or on the activity of total complement.