荧光原位杂交检测TP53缺失与套细胞淋巴瘤患者预后关系

Relation between fluorescence in situ hybridization in detection of TP53 deletion and prognosis of patients with mantle cell lymphoma

目的:分析遗传学异常对侵袭性套细胞淋巴瘤预后的影响。方法:回顾性分析50例伴外周血及骨髓侵犯的套细胞淋巴瘤患者资料。使用荧光原位杂交方法检测患者D13S25/13q14、ATM/11q22、P53/17p13和IGH/CCND1/t(11;14)共4种DNA探针,分析遗传学相关性及预后的影响。结果:Del 13q、Del 11q和Del 17p的发生率分别为36.0%、18.0%和34.0%。单因素分析显示MIPI预后评分系统,Del 13q和Del 17p均为无进展生存的影响因素,而Del 13q、Del 17p和不同治疗方案为总生存的影响因素。结论:P53缺失在套细胞淋巴瘤患者中作为常见的细胞突变,在患者预后中对无进展生存和总生存具有独立的不良预后影响。

Objective：To analyze the impact of genetic abnormalities on prognosis of invasive set of mantle cell lymphoma. Method..We retrospectively analyzed 50 cases of mantle cell lymphoma with peripheral blood and bone marrow invasion. And we detected patients＇ samples using a total of 4 kinds of DNA probe,D13S25/13q14, ATM/ 11q22, P53/17p13 and IGH/CCND1/t（11;14）, by fluorescence in situ hybridization method,in order to analyze the correlation between genetic effect and prognosis. Result.. The incidence of Del 13q, Del 11q and Del 17p were 36.0% ,18.0% and 34.0% ,respectively. Single factor analysis showed that the MIPI prognostic scoring system, Del 13q and Del 17p were impact factors on progression-free survival,and Del 13q, Del 17p and different treatments were impact factors on overall survival. Conclusion： Lack of P53 in mantle cell lymphoma patients as a common set of cell mutation is independent poor factor on progression-free survival and overall survival in the prognosis of patients.