hsa-miR-144在食管鳞癌中的表达及临床意义

The expression and clinical significance of hsa-miR-144 in esophageal squamous cell carcinoma

目的探讨hsa-miR-144在食管鳞癌中的表达情况及其与食管鳞癌临床病理特征及预后的关系。方法采用荧光定量逆转录聚合酶链反应(RT-PCR)对46例食管鳞癌及癌旁正常组织中的hsa-miR-144进行检测。考察hsa-miR-144在食管鳞癌中的表达情况及其在不同性别、年龄、肿瘤大小等临床病理特征中表达水平的差异。分析hsa-miR-144表达与食管鳞癌患者预后间的关系。Kaplan-Meier法和Log-rank考察不同临床病理特征的预后生存率差异。结果 hsa-miR-144在食管鳞癌组织中的相对表达量0.97(0.22,24.48)×10^(-6)低于癌旁正常组织8.60(0.09,258.20)×10^(-6)(Z=2.221,P<0.05)。在食管鳞癌组织中,hsa-miR-144的表达水平淋巴结无转移者高于有转移者;且随着病理分期的升高其表达水平降低(P<0.05),而在性别、年龄、肿瘤大小、肿瘤部位、肿瘤分化程度、肿瘤浸润深度中差异无统计学意义(均P>0.05)。hsa-miR-144表达与食管鳞癌患者生存时间不存在相关性(rs=0.031,P=0.839)。hsa-miR-144高表达组与低表达组的生存时间差异无统计学意义;淋巴结有转移者生存情况较无转移者差,浸润深度较深和病理分期较高者生存情况较差(P<0.05)。结论 hsa-miR-144在食管鳞癌组织中表达下调,与食管癌的淋巴结转移及病理分期有关,hsa-miR-144在食管鳞癌的发生及发展中可能起着抑癌基因作用。

Objective To investigate the expression of hsa-miR-144 in esophageal squamous cell carcinoma, and its relationship with clinicopathological features and prognosis. Methods Reverse transcriptase polymerase chain reaction (RT-PCR) method was used to detect the hsa-miR-144 in 46 cases of esophageal squamous cell carcinoma and adjacent normal tissue. The expression of hsa-miR-144 in esophageal squamous cell carcinoma and its difference in the clinicopatho?logical characteristics including gender, age, and tumor size were investigated. The relationship between the expression of hsa-miR-144 and prognosis of patients with esophageal squamous cell carcinoma was analyzed. Kaplan-Meier method and Log-rank test were used to analyse the differences in survival rates in different pathological characteristics. Results The ex?pression level of hsa-miR-144 was lower in esophageal squamous cell carcinoma 0.97(0.22-24.48)×10-6 than that of adjacent normal tissue 8.60(0.09-258.20)×10-6, the difference was statistically significant (Z=2.221, P<0.05). The expression level of hsa-miR-144 was higher in esophageal squamous cell carcinoma with no lymph node metastasis than that in esophageal squa?mous cell carcinoma with lymph node metastasis (Z=2.758,P<0.05), and the expression level decreased with the increase in the pathological staging (Z=7.737,P<0.05). There were no significant differences in the expression levels of hsa-miR-144 between different gender, age, tumor size, tumor location, tumor differentiation and tumor invasion depth (all P>0.05). There was no correlation between the expression of hsa-miR-144 and prognosis in patients with esophageal squamous cell carcino?ma (rs=0.031, P=0.839). In the survival rate, there was no statistic significance between high expressive of hsa-miR-144 group and low expressive group (P=0.828). The survival rate was lower in patients with lymph node metastasis than that of pa?tients without lymph node metastasis. The survival rates were lower in patients with relatively deep invasion and higher patho?logic stage (P<0.05). Conclusion The expression of hsa-miR-144 is down regulated in esophageal squamous cell carcino?ma, and which is associated with lymph node metastasis and pathological staging of esophageal carcinoma. It shows that hsa-miR-144 may serve as an anti-oncogene in the occurrence and development of esophageal squamous cell carcinoma.