不同浓度硒含量的富硒乳酸菌抑制黄曲霉菌生长以及产毒效果的研究

Study on Se-enriched Lactobacillus with different selenium concentration inhibiting the growth and toxin-producing effects of Aspergillus flavus

为研究不同浓度硒含量的乳酸菌对黄曲霉菌生长及产毒的抑制效果,测定了硒浓度分别为1 0、15、2 0、25μg / m L 的M R S 液体培养基中培养的乳酸菌对黄曲霉菌落生长抑制率、黄曲霉孢子生长抑制率以及黄曲霉产毒量抑制效果.结果显示硒浓度在1 0 -2 5 μg /m L,培养4 8 h 的富硒乳酸菌对于黄曲霉生长的抑制率较乳酸菌组增长了0 . 6 %-9.4%,9 6 h 抑制率达到了 37.1%;培养6 h 的黄曲霉孢子生长抑制率较乳酸菌组增长了 0 . 2 %-6 1 %,24 h 抑制率达到93.6%;产黄曲霉毒素氏抑制率较乳酸菌组提高10.3%-56.6%,2 4 h 抑制率达到了 97.3%.硒浓度达到2 5 滋g/mL的富硒乳酸菌对于黄曲霉生长的抑制率、黄曲霉孢子生长抑制率和产黄曲霉毒素玖抑制率均达到最大.结果提示,富硒乳酸菌对于黄曲霉菌落的生长、孢子萌发以及黄曲霉毒素的产生都有一定程度抑制作用,并且硒浓度达到25μg /m L,抑制效果最好.[

To study inhibitory effect of different concentrations selenium content in se-enriched lactobacillus on the growth and toxin-producing effect of Asporgillus,we determined the effect of Aspergillus colony,flavus and toxin production's growth inhibition rate by Lactobacillus,cultured in MRS liquid medium with 10,15,20,25 μg/m L.The results showed that inhibition ratio of Aspergillus growth effected by se-enriched Lactobacillus cultured in 10 ~ 25 μg/m L sodium selenite concentration for 48 h increased by 0.6% ~ 9.4% and inhibition ratio reached to 37.1% for 96 h,inhibition ratio of Aspergillus flavus for 6 h increased by 0.2% ~ 61% compared with lactic acid bacteria group and inhibition ratio reached to 93.6% for 24 h.The growth inhibition rate of aflatoxin B1 production increased by 10.3% ~ 56.6% compared with lactic acid bacteria group and inhibition ratio reached to 97.3% for 24 h.The growth inhibition rate of Aspergillus colony, flavus and aflatoxin production B1 reached the maximum under the lactic acid bacteria containing 25 μg/m L selenium concentration.These results suggested that se-enriched Lactobacillus presented some inhibition to the growth of Aspergillus flavus coenobium,spore germination and the production of aflatoxin,inhibitory effect was the best when the concentration of Se reached 25 μg/m L.