摘要
本文报道木桔根甲醇提取物（ＭＥＲＡ）及其化学成分对小鼠在体和离体肝脏组织或胞液（ｃｙｔｏｓｏｌ）脂质过氧化的抑制作用。ＭＥＲＡ能够明显增加肝细胞中超氧化物岐化酶和谷胱甘肽过氧化物酶的活性，而对过氧化氢酶活性的影响无统计学意义。其主要成分之一４－甲氧基－１－甲基－２－喹诺酮（ＭＭＱ）有升高ＦｅＣｌ２－抗坏血酸（ＡＡ）－ＡＤＰ中毒小鼠肝细胞中ＳＯＤ活性的作用。从木桔根分离出的各类化合物对离体条件下ＦｅＣｌ２－抗坏血酸，ＣＣｌ４－ＮＡＤＰＨ和ＡＤＰ－ＮＡＤＰＨ等自由基发生系统诱导的大鼠肝细胞中脂质过氧化物质形成有程度不同的抑制作用。在受试化合物中，ＭＭＱ和其衍生物花椒喹诺酮（ｉｎｔｅｇｒｉｑｕｉｎｏｌｏｎｅ）对上述三种自由基发生系统引起的脂质过氧化有较强的抑制作用；在选定的浓度下。
The inhibitory effect of the methanolic extract of the root of Aegle marmelos (MERA) and its constituents on the lipid peroxidation in vivo and in vitro were studied. The results suggested that MERA increased the activities of superoxide dismutase (SOD) and GSH-peroxidase in the liver cytosol of mice, but showed no significant effect on the activity of catalase, and one of its major constituents, 4-methoxy-1-methyl-2-quinolone (MMQ) increased the activity of SOD in liver tissue of mice intoxicated with FeCl2-ascorbic acid (AA)-ADP in vivo. Various constituents isolated from the root of title plant inhibited the lipid peroxidation in rat liver homogenate, which was in vitro induced by FeCl2-ascorbic acid, CCl4-NADPH, or ADP- NADPH. Of the test compounds, MMQ and its derivatives integriquinolone were similar to (-tocopherol in inhibiting MDA production in rat liver microsomes induced by Fe2+-ascorbate, CCl4-NADPH, or ADP-NADPH.
