复发性自然流产患者T细胞中Tim-3和PD-1的表达

Expressions of Tim-3 and PD-1 on T cells in patients with recurrent spontaneous abortion

目的探讨复发性自然流产(RSA)患者T细胞免疫球蛋白黏蛋白-3(Tim-3)及程序性死亡因子-1(PD-1)在T细胞中的表达情况,为其作为治疗RSA的潜在靶点提供理论依据。方法采集22名正常早孕妇女(对照组)及21例RSA患者抗凝外周血,用流式细胞术分别检测T细胞表面分子Tim-3及PD-1的表达情况。结果 RSA患者外周血T细胞中Tim-3^+T细胞比例(1.01%±0.64%)和PD-1^+T细胞比例(40.76%±13.76%)均明显低于对照组(1.85%±0.96%、54.32%±26.65%)(P<0.01、P<0.05);RSA组T细胞中Tim-3^+PD-1^+双阳性T细胞比例(0.59%±0.27%)明显低于对照组(1.37%±0.85%,P<0.001),而Tim-3-PD-1^+T细胞比例(40.17%±13.68%)和Tim-3^+PD-1-T细胞比例(0.43%±0.41%)与对照组(52.95%±26.28%、0.48%±0.31%)比较,差异无统计学意义(P>0.05)。结论 Tim-3^+CD3^+T细胞、PD-1^+CD3^+T细胞及Tim-3^+PD-1^+双阳性T细胞水平降低可能与RSA有关,有作为RSA治疗潜在靶点的价值。

Objective To investigate the expressions of T cell immunoglobin mucin-3(Tim-3) and programmed death-1(PD-1) on T cells in patients with recurrent spontaneous abortion(RSA),and provide a reference for target treatment. Method The expressions of Tim-3 and PD-1 on T cells were determined by flow cytometry in 22 healthy pregnant women(control group) and 21 patients with RSA. Result The proportion of Tim-3^+ T cells in RSA group(1.01%±0.64%) was lower than that in control group(1.85%±0.96%)(P<0.01),and the proportion of PD-1^+ T cells in RSA group(40.76%±13.76%) was lower than that in control group(54.32%±26.65%)(P<0.05). The proportion of Tim-3^+ PD-1^+ T cells in RSA group(0.59%±0.27%)was lower than that in control group(1.37%±0.85%)(P<0.001),while the proportions of Tim-3-PD-1^+ and Tim-3^+ PD-1-T cells(40.17%±13.68% and 0.43%±0.41%) had no statistical significance with those in control group(52.95%±26.28% and 0.48%±0.31%)(P>0.05). Conclusions The expressions of Tim-3^+CD3^+,PD-1^+CD3^+and Tim-3^+ PD-1^+ T cells are correlated with RSA. They can be potential targets for RSA treatment.