纤维桩和金属桩修复后龈沟液中炎性因子和蛋白酶含量、氧化应激程度的测定

Detection of inflammatory factor and protease content as well as oxidative stress level in gingival crevicular fluid after fiber post and metal post repair

目的:研究纤维桩和金属桩修复后龈沟液中炎性因子、蛋白酶的含量以及氧化应激程度。方法:选择在我院接受桩核修复的大面积牙体缺损患者60例进行研究,根据桩核修复材料不同分为纤维桩组(n=28)和金属桩组(n=32),测定龈沟液中炎性因子、蛋白酶分子、氧化应激分子的含量。结果:纤维桩组龈沟液中IL-12p35、IL-12p40、CXCL16、NFATc1、RANK、MMP-1、MMP-8、MMP-13、P.g-TLP、EA、ROS、MDA、LPO、8-OHdG的含量低于金属桩组;纤维桩组龈沟液中IL-4、IL-13、SOD、GP-x、CAT的含量高于金属桩组;两组血清中IL-12p35、IL-12p40、CXCL16、NFATc1、RANK、IL-4、IL-13的含量无差异。结论:纤维桩对牙周组织的创伤较小,龈沟液中促炎因子、蛋白酶分子以及过氧化产物含量较低。

Objective:To study inflammatory factor and protease content as well as oxidative stress level in gingival crevicular fluid after fiber post and metal post repair.Methods:A total of 60 cases of patients with large tooth defect who received post-core restoration in our hospital were selected and divided into fiber post group(n=28)and metal post repair group(n=32)according to different post-core restoration materials,and the content of inflammatory factors,proteases,oxidative stress molecules in gingival crevicular fluid were detected.Results:IL-12p35,IL-12p40,CXCL16,NFATc1,RANK,MMP-1,MMP-8,MMP-13,P.g-TLP,EA,ROS,MDA,LPO and 8-OHdG contents in gingival crevicular fluid of fiber post group were lower than those of metal post group;IL-4,IL-13,SOD,GP-x and CAT content in gingival crevicular fluid of fiber post group were higher than those of metal post group;serum IL-12p35,IL-12p40,CXCL16,NFATc1,RANK,IL-4and IL-13 content of two groups were not significant different.Conclusion:Fiber post causes less trauma to periodontal tissue,and the content of pro-inflammatory factors,proteases and peroxidation products in gingival crevicular fluid were lower.