不同生长期酸枣叶中多类型资源性化学成分的积累变化与分析评价

Dynamic Analysis of Nucleosides,Amino Acids and Flavonoids in Leaves of Ziziphus jujuba var.spinosa Collected in Different Growth Period

目的:研究不同生长期酸枣叶中多类型资源性化学成分的动态变化规律,为酸枣叶适宜采收期的确定及资源高效利用提供依据。方法:采用UPLC-TQ/MS法和HPLC法分别建立酸枣叶中19种核苷类和23种氨基酸类,以及黄酮类成分芦丁、金丝桃苷、异槲皮苷的分析方法,并比较不同生长期酸枣叶中上述组分的组成及含量变化趋势。结果:不同生长期酸枣叶中多类型资源性化学成分含量差异较大。其中,核苷类化学成分随生长期变化总体含量表现为先升高后降低,再升高后再降低的双峰变化趋势;氨基酸类化学成分总量总体表现为先升高后降低的变化趋势;黄酮类化学成分总体表现为含量由高渐低的变化趋势。3类化学成分均以6月28日所采集样品含量相对较高。结论:综合分析,酸枣叶以制茶为主要利用目的其适宜采收期应为6月下旬。本研究结果为酸枣叶适宜采收期的确定提供了科学依据,也为酸枣叶资源的有效利用提供了数据支撑。

Objective：To investigate the dynamic changes of multiple ingredients in Ziziphus jujuba var.spinosa leaf （ZSL） collected in different growth period,and provide the data for selection of the suitable harvest time of ZSL.Methods：UPLC-TQ MS and HPLC methods were used to determine the contents of 19 nucleosides and 23 amino acids as well as rutin,hyperoside and isoquercitrin in ZSL and their contents in the samples collected in different growth periods were compared.Results：The content differences of nucleosides,amino acids and flavonoids in ZSL collected in different growing periods were greatly obvious.Among them,the content of nucleosides generally exhibited two peaks with the leaf growth.Amino acids content showed a trend that increased firstly,then decreased.For flavonoids,a gradually reducing trend from the high level of their whole content was found.The total content of these three types constituents were relatively high in samples collected in June 28th.Conclusion：Given the above results,the suitable harvesting time for ZSL should be in late June when it was mainly used to prepare the tea.The results of this study provide a scientific basis for selecting the suitable harvesting period for ZSL as well the data for the efficient utilization of ZSL resource.