A Molecular Approach to Identification of the Chinese Drug“pu Gong Ying”(Herba Taraxaci)and Six Adulterants byDNA Fingerprinting Using Random Primed PolymeraseChain Resaction(PCR)

中药材蒲公英及其混淆品的DNA指纹鉴别研究

DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbitrarily primed polymerase chain reaction(AP-PCR)and random amplified polymorphic DNA(RAPD).Distinctive,reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long(20 and 24 mer)and one short(10 mer)randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover,the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore,AP-PCR and RAPD methods can be used for identifying Chinese drugs.

采用分子生物技术包括任意引物聚合酶链式反应（ＡＰ－ＰＣＲ）和随意扩增多态性ＤＮＡ（ＲＡＰＤ）方法扩增蒲公英及其六种土公英混淆品的基因组ＤＮＡ，获得清晰可靠的ＤＮＡ指纹图谱。根据琼脂糖胶上显示的ＤＮＡ带型差异可鉴别蒲公英和土公英混淆品。