基于电润湿的液滴捕获与分选方法

Droplet Trapping and Sorting Based on Electro-wetting

在微流控芯片中制作了进行电润湿操控的微电极。利用微流控芯片的微沟道和电润湿的作用,将高通量和精确控制这两个优点结合起来。研究了液滴的半径,移动速度和电极结构与捕获电压之间的关系,用112 V电压捕获了半径为60μm的液滴。利用电润湿实现了对液滴的分选,发现液滴的半径,移动速度和驱动的方式,会影响分选所需的电压。利用42 V电压实现了对半径为60μm的液滴捕获。利用电润湿作用,我们成功将包裹He PG2细胞的海藻酸钠液滴分选出来。

Precise manipulation of individual drops is crucial in many microfluidic applications. By incorporating integrated electro- wetting on dielectric （EWOD） into channel- based discrete microfluidics, the high throughput from channel-based microfluidics was combined with individual drop control from electrical actuation. In the experiment of trapping individual droplet, the relationship among the droplet radius, velocity, the electrodes structure and demanded voltage for successful trap was investigated. Droplets with a radius of 60 μm were trapped using 112 V alternating voltage. The experiment of sorting presented that the required voltage was influenced by the radius, velocity of the droplet and the actuation mode. Droplets with a radius of 60 μm were sorted using 42 V alternating voltage. Droplet containing HePG2 cells were sorted by utilizing channel-based microfluidics and electro-wetting.