银杏叶提取物与华法令协同抗凝影响的研究

Effect of ginkgo bibola leaves extract on coagulation status in rabbits with anticoagulant

目的通过观察银杏叶提取物注射液（Egb761）对抗凝家兔PLT、PT、APTT、血小板凝血功能的作用,探讨Egb761对华法令抗凝的影响.方法48只家兔随机分成两大组,第1大组服华法令抗凝,使PT达到正常值的1.5～2.5倍（国际标准化比值为1.8～3.7）,第2大组服华法令抗凝使PT达到正常值的2.5～3.5倍（国际标准化比值为3.7～6.0）,每大组又分成对照组（耳缘静脉注射0.9％氯化钠注射液0.3ml/kg）,小剂量组（耳缘静脉注射银杏叶提取物注射液0.3ml/kg）,中剂量组（耳缘静脉注射银杏叶提取物注射液1.5ml/kg）和大剂量组（耳缘静脉注射银杏叶提取物注射液3.0ml/kg）,每组6只（雌雄对半）,连续给药7d,于给药的第7天静脉注射金纳多,1.5h后自耳缘动脉采血5ml,进行PLT、PT、APTT、血小板3因子有效性（PF3aT）等检测.结果Egb761对抗凝家兔的PT、APTT、PLT均无影响（均P＞0.05）,但影响血小板3因子（PF3）,使PF3aT的第3管较第4管延长（P＜0.05）,且长INR实验组的影响明显大于短INR组.结论Egb761通过影响血小板活化,进而影响PF3,使机体凝血功能缺陷,并与华法令产生协同作用,使机体凝血功能缺陷更明显,结果使机体处于易出血状态,从而影响华法令抗凝治疗.

Objective To investigate the effect of extract of ginkgo bibola leaves （Egb） on coagulation status in rabbits with anticoagulant. Methods Forty eight rabbits were divided randomly into two groups （n=24 in each group）. Warfarin was given in group 1 to achieve 1.8~3.7 times INR of normal rabbits; and given in group 2 to achieve3.7~6.0 times INR. Each group was further divided into 4 subgroups with 6 animals in each, including three Egb groups and one control group. Intravenous infusion of 0.3ml/kg NS was given to the rabbits in control group, and 0.3ml/kg, 1.5ml/kg, 3ml/kg Egb761 were given to the rabbits in three Egb groups for 7 days, respectively. After 1.5 h of injection on d7, the blood samples were collected, and PLT, PT, APTT, PF3aT were examined. Results Egb761 had no effect on PLT, PT, APTT of anticoagulant rabbits（P 〉0.05）; while it affected PF3aT levels （P〈0.05）, the effect was more marked in group 1 than that in group 2. Conclusion Injection of ginkgo bibola leaves extracts may affect anticoagulation therapy of warfarin in rabbits.