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长链非编码RNA RP11-191L9.4促进前列腺癌PC-3细胞的迁移和侵袭 被引量：2

Effection of lncRNA RP11-191L9.4 on the migration and invasion of prostate cancer cell PC-3
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摘要 目的:探讨长链非编码RNA RP11-191L9.4对前列腺癌PC-3细胞增殖、迁移及侵袭能力的影响。方法:荧光定量PCR检测RP11-191L9.4 siRNA在PC-3细胞中的敲除效率;通过MTT实验和克隆形成实验观察在PC-3细胞中低表达RP11-191L9.4对其增殖的影响;通过transwell细胞迁移实验检测敲低RP11-191L9.4的表达对PC-3细胞的迁移能力影响;通过Matrigel侵袭实验检测敲低RP11-191L9.4的表达对PC-3细胞侵袭能力的影响。结果:向前列腺癌细胞系PC-3中转染RP11-191L9.4 siRNA 48 h后,q PCR检测PC-3细胞中RP11-191L9.4明显低表达;MTT及克隆形成实验结果显示,敲低RP11-191L9.4的表达能显著抑制PC-3细胞的增殖;transwell迁移实验结果显示,敲低RP11-191L9.4的表达能减弱PC-3细胞的迁移能力;Matrigel胶细胞侵袭实验结果显示,敲低RP11-191L9.4的表达能减弱PC-3细胞的侵袭能力。结论:转染RP11-191L9.4 siRNA能显著抑制PC-3细胞中RP11-191L9.4的表达,而敲低RP11-191L9.4的表达可显著抑制前列腺癌细胞PC-3增殖、迁移及侵袭能力。 Objective： To explore the effect of lncRNA RP11-191L9. 4 on the proliferation,migration and invasion ability of prostate cancer PC-3 cells. Methods： q PCR assay was used to confirm the low expression level of RP11-191L9. 4 after the transfection of RP11-191L9. 4 siRNA. MTT assay and colony-forming unit assay were performed to evaluate the effect of RP11-191L9. 4 knock-down on PC-3 cell proliferation. Transwell migration assay was used to evaluate the effect of RP11-191L9. 4 knock-down on the migration ability of PC-3 cells. Matrigel invasion assay was used to evaluate the effect of RP11-191L9. 4 knock-down on the invasion ability of PC-3 cells. Results： The expression level of RP11-191L9. 4 was significantly knocked down by the transfection of siRNA. MTT assay and colony forming unit assay showed that reducing the expression of RP11-191L9. 4 can significantly weaken the proliferation ability of PC-3 cells. The result of transwell migration assay indicated that reducing the expression of RP11-191L9. 4 in PC-3 cells obviously suppressed its migration ability. The result of Matrigel invasion assay demonstrated that reducing the expression of RP11-191L9. 4 significantly suppressed the invasion ability of PC-3cells. Conclusion： RP11-191L9. 4 level in PC-3 cells can be efficiently knocked down with the transfection of RP11-191L9. 4 siRNA,and knock down the expression level of RP11-191L9. 4 can significantly suppress the ability of proliferation,migration and invasion in PC-3 cells.
作者 王京 黄烨清 许斌 陈明
机构地区 东南大学医学院 东南大学附属中大医院
出处 《东南大学学报（医学版）》 CAS 北大核心 2016年第3期305-310,共6页 Journal of Southeast University(Medical Science Edition)
基金 国家自然科学基金(81370849 81300472 81202034) 临床医学科技专项--新型临床诊疗技术攻关基金(BL2013032) 教育部博士点基金(20120092120071) 江苏省自然科学基金(BK2012336) 南京市科技发展项目基金(201201053) 东南大学新教师基本科研项目基金(3290002402)资助项目
关键词 lncRNA RP11-191L9.4 前列腺癌 细胞增殖 细胞迁移 肿瘤侵袭 lncRNA RP11-191L9 4 prostate cancer cell proliferation cell migration tumor invasion
分类号 R737.25 [医药卫生—肿瘤] R-33 [医药卫生]
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参考文献17

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东南大学学报（医学版）
2016年 第3期

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