p-STAT3蛋白在增生性瘢痕中的表达及意义

Expression of STAT3 protein in hypertrophic scar and its significance

目的检测JAK2/STAT3信号通路中STAT3蛋白在人增生性瘢痕中的表达,探讨其在增生性瘢痕中的作用及意义。方法采用免疫组织化学方法和Western blot检测方法分别检测32例增生性瘢痕和22例正常皮肤组织中p-STAT3的表达情况。结果(1)免疫组织化学检测显示,p-STAT3蛋白在增生性瘢痕及正常皮肤组织中均有表达,染色阳性物质主要位于细胞质,少部分位于细胞核,在表皮和真皮层均有表达,在增生性瘢痕组织中表达量均高于正常皮肤组织,差异具有统计学意义(P=0);(2)Western blot检测结果显示,增生性瘢痕中p-STAT3蛋白的表达均较正常皮肤高,差异具有统计学意义(P<0.05)。结论增生性瘢痕中p-STAT3呈现高表达,提示JAK2/STAT3信号通路异常激活可能在增生性瘢痕发生过程中起重要作用。

Objective To investigate the expression of STAT3 protein of JAK2/STAT3 pathway in hypertrophic scars and its significance.Methods The expression of p-STAT3 in 32 cases of hypertrophic scar and 22 cases of normal skin were detected by immunohistochemistry （IHC） and Western blot.Results （1） IHC showed that p-STAT3 protein was expressed in both hypertrophic scar and normal skin.Normal skin tissue staining substances are mainly located in the cytoplasm,with a small part in the nucleus.The level of p-STAT3 protein in hypertrophic scars was higher than that in the normal skin,the difference had a statistical significance （P =0）;（2） Western blot showed that the expression of p-STAT3 in the hypertrophic sear fibroblasts was higher than that in the normal human skins;the difference had a statistical significance （P ＜ 0.05）.Conclusion The strong expressions of p-STAT3 in hypertrophic scars indicated that the abnormal activity of the JAK2/STAT3 pathway may play an important role in the occurrence of the hypertrophic scar.