摘要
顺铂淬灭红细胞膜蛋白的内源性荧光存在浓度依赖关系，这种性质可用以测定铂与膜蛋白的作用程度。在氯离子和硫酸根存在下，顺铂的淬灭作用减弱。二者作用也与ｐＨ有关，低ｐＨ条件作用较强。根据顺铂对醋酸汞荧光素标记膜蛋白的荧光的影响，认为铂与膜蛋白巯基结合。基于共存阴离子的影响，讨论了顺铂，膜蛋白可能的反应机制。
The
interaction between cisplatin and erythrocyte membrane proteins was
studied based on the quenching effect of cisplatin on the intrinsic
fluorescence of proteins.A concentration-dependent quenching effect
was observed.The presence of chloride and sulphate weakens the effect
significantly.A pH-dependence was also noted with a stronger effect
in acidic solution. The nature of the interaction is considered to be
platinum-thiol group binding according to the effect of cisplatin on
the fluorescence of FMA labeled membrane. The mechanism of the
cisplatin-protein interactions was discussed based on the effect of
coexisting anion
基金
国家自然科学基金
国家教委基金资助课题
