摘要
从湖北恩施大蒜（Allium sativum L.）中分离出分子量为1.5×10^4的含硒多糖，经G25－G200葡聚糖凝胶色谱柱多次精制，得到一纯品S -GPS.,应用比旋光度法、凝胶过滤法及高效液相色谱法检测其化学均一性（分散度为2.1436），通过高效液相色谱及两个系统的纸层析测定其组成为与硒有键合状态的甘露聚糖。生物活性实验表明Se-GPS有清除活性氧自由基的能力；对SiO2所引起的细胞损伤有保护作用；在细胞特异性病变抑制实验中显示出较好的结果；对人巨细胞病毒所形成的空斑抑制率为38.6%。
A Se—containing polysaccharide(Se-GPS),with average molecular weight of 1.5×10~4 was isolated from Hubei Enshi garlic(Allium sativum L.)and purified by means of Sephadex G25 to G200 chromatographies.Its chemical homogeneity and composition were studied by optical rotation,HPLC and paper chromatographical methods.The abilities of scavenging active oxygen free radicals and inhibiting the damage of erythrocyte membrane by SiO_2 were established by spin trapping ESR studies.Se-GPS was shown to inhibit human cytomegalovirus by plague reduction assays and cytopathogenic effect tests.
