一种新型B群链球菌显色培养基的应用及效果评定

The application and performance evaluation of a novel color culture medium for group B Streptococcus

目的评价一种新型的B群球菌显色平板的性能。方法收集2014年5月-12月从本院门诊及住院患者的各类临床样本中分离的细菌,共405株,其中B群链球菌105株,菌种的鉴定采用VITEK 2 Compact全自动微生物分析仪,同时采用B群链球菌显色培养基进行重新鉴定。结果使用B群链球菌显色平板对405株临床标本进行检测,105株B群链球菌中检测为阳性的有103株,2株为阴性。300株非B群链球菌菌株中检测为阳性的有9株,其余291株为阴性。B群链球菌显色平板检出B群链球菌的灵敏度为98%,特异性为97%,阳性预测值为91.96%,阴性预测值为99.32%。结论本实验所用B群链球菌显色平板对临床标本中的B群链球菌检测性能较好,适用于妇产医院或基层医院中B群链球菌的分离和鉴定。

Objective To evaluate the application and performance of a novel color culture medium for group B Streptococcus（ GBS）. Methods From May to December in 2014,405 strains of bacteria isolated from the various specimens of patients at the First Affiliated Hospital of Wenzhou Medical University were collected,including 105 strains of GBS. The identification of bacteria were determined by VITEK 2 Compact microbiology analyzer,and GBS color culture medium was used for re-identification. Results 405 clinical strains of bacteria were detected with GBS color culture medium,and 103 out of 105 group B Streptococcus strains were identified as positive,with the rest 2 strains negative. 9 out of 300 non GBS strains were positive,and the remained 291 strains were negative. The GBS color culture medium test showed that the sensitivity and specificity of B group Streptococcus was respectively 98% and 97%,and the positive predictive value was 91. 96%,the negative predictive value was99. 32%. Conclusion This GBS color culture medium used in this experiment showed better detection performance of clinical group B Streptococcus specimens,which is suitable for isolation and identification of group B Streptococcus in maternity hospital or local hospital.