白细胞介素-6基因启动子区-572位点多态性与EV71感染疾病严重程度的相关性分析

Association of IL-6-572G/C polymorphism with severity of enterovirus 71 infection

目的探讨白细胞介素-6（Interleukin-6,IL-6）基因启动子区-572位点单核苷酸多态性（single nucleotide polymorphism,SNP）与儿童肠道病毒71型（enterovirus 71,EV71）感染疾病严重程度的相关性。方法提取87份EV71重症感染患者和57份无症状隐性感染儿童血液样本基因组DNA,进行PCR扩增及测序,检测IL-6基因启动子区-572位点多态性,利用Logistic回归分析基因型频率。结果 EV71重症感染患者IL-6基因型频率与对照组相比,差异有统计学意义（OR=2.605,95%CI：1.117～6.074,P=0.027）。IL-6多态性与EV71感染严重程度有关（OR=2.411,95%CI：1.236-4.706,P=0.010）。结论 IL-6多态性与中国儿童EV71重症感染可能具有相关性,对确定影响疾病严重程度的宿主遗传因素和增强治疗效果具有重要意义。

Objective To investigate the association of single nucleotide polymorphism（SNP） of site 572 in promoter region of interleukin-6（IL-6-572 G / C） with the severity of enteroviurs（EV） 71 infection in children. Methods Blood samples of 87 EV71-infected patients and 57 asymptomatic children with latent infection were collected, from which genomic DNAs were extracted, amplified by PCR and sequenced, then tested for the polymorphism of IL-6-572 G / C, and analyzed for genotype frequency by Logistic regression. Results The frequency of IL-6 genotype in patients severely infected with EV71 showed significant difference with that in control group（OR = 2. 605,95% CI：1. 117 ~ 6. 074,P =0. 027）. The polymorphism of IL-6（OR = 2. 411,95% CI：1. 236 ~ 4. 706,P = 0. 010）was found to be associated with the severity of EV71 infection. Conclusion IL-6 polymorphism may be associated with an increased risk of severe EV71 infection in Chinese children, which is of an important significance in determination of the host genetic factors influencing the severity and enhancement of curative effect of EV71 infection.