人面果叶子的化学成分研究

Chemical constituents from leaves of Garcinia xanthochymus

采用正、反相硅胶柱色谱及半制备高效液相色谱等方法从人面果叶子95%乙醇提取物的乙酸乙酯部位分离得到15个化合物,分别鉴定为5,7,4'-trihydroxy-6-(3-hydroxy-3-methylbutyl)-flavone(1)、1,5-二羟基-3-甲氧基口山酮(2)、1,3-二羟基-5-甲氧基口山酮(3)、山柰酚(4)、二氢山柰酚(5)、3,24,25-trihydroxytirucall-7-ene(6)、对羟基桂皮酸(7)、异香草酸(8)、(Z)-2-(2,4-dihydroxy-2,6,6-trimethylcyclohexylidene)(9)、volkensiflavone(10)、藤黄双黄酮(11)、3,8″-双柚皮素(12)、白果素(13)、fukugiside(14)和GB2a glucoside(15)。化合物1为新化合物,命名为5,7,4'-trihydroxy-6-(3-hydroxy-3-methylbutyl)-flavone。化合物5,6,9,13为首次从藤黄属植物中分离得到。化合物4,7~8,10~12,14~15为首次从人面果中分离得到。研究了10个化合物对α-淀粉酶的抑制作用,结果显示,化合物13对α-淀粉酶具有抑制活性,化合物13和阿卡波糖对α-淀粉酶的半数抑制浓度分别为8.12,4.32μmol·L^(-1)。

The constituents were isolated and purified by the silica gel and semi-preparative HPLC, and their structures were elucidated by NMR spectral and MS data. Fifteen compounds were isolated from the ethyl acetate fraction of 95% ethanol extract from the leaves of Garcinia xanthochymus, and identified as 5, 7, 4＇-trihydroxy-6 - （3-hydroxy-3-methylbutyl） -flavone （ 1 ）, 1,5-dihydroxy-3-methoxyxanthone （2）, 1, 3-dimethoxy-5-hydroxy xanthone （ 3 ）, kaempferol （ 4）, （ 2S, 3S） -trans-dihydrokaempferol （ 5 ）, 3, 24, 25-trihydroxytirucall-7-ene （ 6 ）, 4 -hydroxycinnamic acid （ 7 ）, isovanillic acid （ 8 ）, （ Z ） -2 - （ 2,4 -dihydroxy-2, 6, 6-trimethylcyclohexylidene ） acetic acid （9）, volkensiflavone （ 10）, morelloflavone （ 11 ）, 3, 8＂-biapigenin （ 12）, bilobetin （ 13）, fukugiside （ 14 ）, GB2a glucoside （15）. Compound 1 is a new compound, compounds 5, 6, 9 and 13 are isolated from the genus Garcinia for the first time, and compounds 4, 7-8, 10-12, 14 and 15 are firstly found from this plant, α-Amylase inhibitory activities of 10 compounds were determined using starch azure as the substrate, and the results show that compound 13 has the inhibitory activities against a-amylase, IC50 values of compound 13 and acarbose are 8. 12, 4. 32 μmol . L^-1 respectively.