Septin-9在人肾细胞癌组织的表达及临床意义

Expression of septin - 9 in human renal cell carcinoma and clinical significance

目的观察Septin-9在人肾细胞癌（RCC）组织中蛋白和基因的表达，探讨Septin-9在RCC肿瘤微血管形成中的作用。方法分析我院60例手术切除的RCC患者的临床病理资料，以Westernblot法和实时定量聚合酶链反应（Real-timePCR）定量分析Septin-9的蛋白和mRNA的表达，行CD105标志RCC的微血管密度（MVD），研究Septin-9在RCC中的表达及与MVD的相关性。结果（1）RCC组织与癌旁组织均有Septin-9基因和蛋白表达，在不同病理级别的RCC组织中Septin-9基因表达量为1．258±0．372、1．339±0．408、1．502±0．476和1．689±0．511，蛋白表达量为1．325±0．315、1．597±0．376、1．634±0．513和1．805±0．572，高于癌旁组织和正常组织（P〈0．05）；随着RCC病理级别的增加，基因及蛋白的表达增强；（2）RCC组织中CD105阳性表达率为76．67％（46／60），癌旁组织阳性表达率为13．33％（2／15），差异有统计学意义（P〈0．05）；癌旁组织中MVD值为12．38±2．05，在不同病理级别的RCC组织中MVD值分别为21．59±4．39、29．84±7．43、38．61±6．27、52．18±6．22，MVD值随肿瘤级别的增加而增加。结论Septin-9的表达同RCC的病理分期密切相关，可能通过干预肿瘤微血管形成参与RCC的临床进展。

Objective To study the expression of septin - 9 protein and gene in renal cell carcino- ma （RCC） and normal renal tissues and reveal the role of septin - 9 gene in the process of angiogenesis in renal tumors. Methods The clinicopathologic data of 60 cases of RCC freshly excised in our hospital were retrospectively analyzed. The expression of septin -9 protein and mRNA was detected by Western blotting and real - time quantitative polymerase chain reaction （ Real - time PCR）. Tumor microvessel density （MVD） in CD105 - labeled RCC was measured. The correlation between the expression of septin - 9 in RCC and MVD was analyzed. Results （1） RCC tissues and adjacent cancer tissues both had the expres- sion of septin - 9 gene and protein. The gene expression of septin - 9 in different pathological grades of RCC was 1. 258 ±0. 372, 1. 339 ±0. 408, 1. 502 ±0. 476, 1. 689 ±0. 511, and the protein expression was 1. 325 ＋ 0. 315, 1. 597 ± 0. 376, 1. 634 ＋ 0. 513, 1. 805 ± 0. 572, which was significantly higher than that in tumor adjacent tissues and normal tissue （P 〈 0. 05 ）. With the increases in pathological grades of RCC, the expression of septin - 9 gene and protein was up - regulated. （2） The positive expression rate of CD105 in RCC tissue was 76. 67% （46/60）, and 13.33% （2/15） in cancer adjacent tissue with the difference being statistically significant （P 〈 0. 05）. The MVD in the cancer adjacent tissue was 12. 38 ± 2. 05. MVD in different pathological grades was 21.59 ± 4. 39, 29. 84 ± 7. 43, 38.61 ± 6. 27, and 52. 18 ± 6. 22 respectively. The MVD was increased with the increases in the pathological grades. Conclusion The expression of septin - 9 played an important role in the development of human RCC probably by interfering with the tumor microvascular formation.