血管活性肠肽对前列腺癌DU145细胞迁移侵袭及上皮-间充质转化的影响

Effect of vasoactive intestinal peptide on cell migration and invasion and epithelial - mesenchymal transition in prostate cancer DU145 cells

目的观察血管活性肠肽（VIP）对前列腺癌（DU145）细胞迁移、侵袭的影响并探讨其在上皮-间充质转化（EMT）过程中机制。方法用浓度分别为0、25、50、100μmol／L的VIP处理DU145细胞，采用反转录聚合酶链反应（RT-PCR）和Westernblot检测24h时DU145细胞中E-钙黏蛋白（E—cadherin）、波形蛋白（Vimentin）、SnailmRNA和蛋白的表达；采用划痕实验和Transwell迁移、侵袭实验检测48h时DU145细胞运动、迁移、侵袭能力变化。结果随着VIP浓度的增加，在24h时，细胞中E—cadherin、Vimentin、SnailmRNA表达增加（0．897±0．053、0．715±0．041、0．521±0．036、0．343±0．029；0．586±0．061、0．827±0．089、1．329±0．107、1．764±0．135；0．645±0．055、0．917±0．074、1．383±0．097、1．725±0．124），差异有统计学意义（P〈0．05）；在48h，划痕实验显示DU145运动能力逐渐增加，Transwell迁移、侵袭实验显示穿越小室的细胞个数也增多[（76．7±5．8）、（104．5±6．7）、（128．34-10．4）、（147．7±12．6）个；（84．2±6．4）、（118．3±10．3）、（142．1±13．7）、（173．6±15．4）个]，差异均有统计学意义（P〈0．05）。结论VIP可通过EMT促使前列腺癌DU145细胞运动、迁移及侵袭能力增加。

Objective To observe the effect of intestinal peptide on cell migration and invasion of the DU145 cells, and explore the mechanism of epithelial - mesenchymal transition. Methods DUI45 cells were treated with VIP at concentrations of 0, 25, 50 and 100 μmol/L, respectively. E -cadherin, Vimentin, and Snail mRNA and protein were detected by reverse transcriptase -polymerase chain reaction （ RT - PCR） and Western blotting respectively at 24 h. The cell mobility, migration and invasion ability of DU145 cells were respectively measured by wound - healing assay, Transwell migration and invasion assays at 48 h. Results With the increases of concentrations of VIP for 24 h, the expression levels of E - cadher- in, Vimentin, and Snail mRNA in PC -3 cells were increased at 24 h （0. 897 ±0. 053, 0. 715 ±0. 041, 0.521±0.036, 0.343 ±0.029; 0.586 ±0.061, 0.827 ±0.089, 1.329 ±0.107, 1.764 ±0.135; 0. 645 ±0. 055, 0. 917 ±0. 074, 1. 383 ±0. 097, 1. 725 ±0. 124;P 〈0. 05）. Meanwhile, the cell mobility was increased, the number of migrative cells and invasive cells was significantly increased gradually with the increased concentrations of VIP [ （76.7 ± 5.8 ）, （ 104. 5 ± 6. 7 ）, （ 128.3 ± 10.4 ）, （ 147.7 ± 12. 6 ） cells; （84. 2 ±6.4）, （ 118.3 ± 10. 3）, （ 142. 1 ± 13.7）, （ 173.6 ± 15.4） eells;P 〈0. 01 ]. Conclusion VIP can increase ability of movement, migration and invasion of prostate cancer DU145 cells by epithelial - mesenchymal transition.