缺氧对泡球蚴原头节血管内皮生长因子、微血管密度、半胱氨酰天冬氨酸特异性蛋白酶-3表达的影响

Influence of hypoxia on the expression of vascular endothelial growth factor, microvessel density- CD34 and cysteinyl aspartate-specific protease-3 in echinococcus multilocularis protoscoleces

目的观察缺氧对泡球蚴原头节血管内皮生长因子（VEGF）、微血管密度（MVD）-CD34、半胱氨酰天冬氨酸特异性蛋白酶。3（Caspase-3）表达的影响。方法将泡球蚴原头节与肝癌HepG2细胞共培养1周后取原头节平均分为3组，即正常培养组（A组）、二甲基亚砜（DMSO）溶剂对照组（B组）、去铁敏诱导组（C组），继续培养24h后伊红染色检测头节的活力，酶联免疫吸附试验（ELISA）法检测缺氧诱导因子-1α（HIF—1α）、VEGF、CD34的表达，可见光光度比色法检测Caspase-3的活性。结果继续培养24h后，3组原头节活力差异无统计学意义（P〉0．05）；A、B、C组泡球蚴原头节的HIF-1α的表达量分别为（852．00±124．41）、（806．20±118．51）、（1168．20±93．59）ng／L；VEGF的表达水平分别为（158．28±29．24）、（157．49±26．88）、（222．36±18．87）ng／L；CD34的表达水平分别为：（605．40±49．95）、（592．20±51．91）、（873．60±61．15）ng／L；Caspase-3的释放量分别为（14．90±1．06）、（15．54±1．17）、（20．54±2．25）mmol／L；C组与A组和B组比较，差异有统计学意义（P〈0．05）。结论模拟体内缺氧微环境，可上调泡球蚴原头节VEGF、CD34、Caspase．3表达，血管新生可能是泡球蚴侵袭性生长的重要原因之一。

Objective To explore the effects of hypoxia on the expression of vascular endothelial growth factor （ VEGF）, CD34 and cysteinyl aspartate-specific protease-3 （ Caspase-3 ） in Echinococcus multilocularis protoscoleces. Methods Culture the Echinococcus muhilocularis protoscoleces and HepG2 cell for one week, and then divide the protoscoleces into three groups, that is the normal cultured control group（group A）, dimethyl sulfoxide （DMSO） solvent control group （group B）, deferoxamine induced group （ group C）. Continue to culture 24 hours and eosin stain to test the vitality of the protoscoleces, tests the expression of hypoxia inducible factor-lot （HIF-lot）, VEGF, CD34 and Caspase-3. Results Contin- ued to culture 24 hours, the vitality of three groups were not statistically significant （P 〉 0. 05） ; The ex- pression quantity of HIF-1α of each group was （852. 00 ± 124.41 ）, （ 806. 20 ± 118.51 ）, （ 1168.20 ± 93.59） ng/L; the VEGF was （ 158.28 ±29. 24）, （ 157.49 ±26. 88） （222. 36 ± 18.87） ng/L; the CD34 was （605.40 ±49. 95）, （592. 20 ±51.91）, （873.60 ±61.15） rig/L, and the Caspase-3 of each group was （ 14. 90 ± 1. 057）, （ 15.54 ± 1.17）, （20. 54 ±2. 25） mmol/L, group C compared with group A and group B, the difference had statistical significance （P 〈 0. 05 ）. Conclusion Hypoxia in vitro can in- crease the expression quantity of VEGF, CD34, Caspase-3, and angiogenesis may be one of the important reasons that lead to the invasive growth of echinococcus multilocularis.