牦牛角性状候选基因的筛选

Screening of Candidate Genes for Horn Trait of Yak

本研究旨在筛选牦牛角性状候选基因,为角发育机制的研究和无角牦牛的培育提供科学依据。采集牦牛角发育初期胎龄90d的有角和无角牛的角基及额部皮肤组织,利用实时荧光定量PCR技术(qRT-PCR),比较牛角性状候选基因OLIG1、OLIG2、IFNAR1、IFNAR2、C1 H21orf62、GCFC1、IFNGR2、SYNJ1、IL10RB、GART、RXFP2、FOXL2、TWIST1、TWIST2、ZEB2、E-cadherin和P-cadherin mRNA在角基间及皮肤间的相对表达量。结果显示,候选基因OLIG1、IFNAR1、C1 H21orf62、SYNJ1、IL10RB、GART、RXFP2、TWIST2和E-cadherin在有角和无角牦牛角基间和皮肤间转录量差异不显著(P>0.05)。IFNGR2、FOXL2、TWIST1和P-cadherin在有角和无角牦牛角基间转录量差异显著(P<0.05)。IFNAR2和GCFC1在有角和无角牦牛角基间和皮肤间转录量差异都显著(P<0.05)。OLIG2和ZEB2在有角和无角牦牛角基间转录量差异极显著(P<0.01)。推测OLIG2和FOXL2可能是牛角性状的关键候选基因,同时ZEB2、TWIST1和IFNGR2基因可能对牛角的形成具有重要作用,而P-cadherin基因可能参与了牛角的发育。

This study screened the candidate genes of horn trait which provides scientific basis for the horn developmental mechanism and the polled yak breeding.The horn bud and forehead skin tissue from horn and polled yaks with 90 dgestational age were collected,and the mRNA different expression of OLIG1,OLIG2,IFNAR1,IFNAR2,C1 H21orf62,GCFC1,IFNGR2,SYNJ1,IL10 RB,GART,RXFP2,FOXL2,TWIST1,TWIST2,ZEB2,E-cadherin,P-cadherin genes were analyzed using real-time quantitative PCR（qRT-PCR）.The results showed that expressions of OLIG1,IFNAR1,C1 H21or f62,SYNJ1,IL10 RB,GART,RXFP2,TWIST2 and E-cadherin genes showed no significant differences in horn and skin between horned and polled yaks（P〉0.05）.There were significant difference at transcript levels of IFNGR2,FOXL2,TWIST1 and Pcadheringenes in horn buds between horned and polled yaks（P〈0.05）.IFNAR2 and GCFC1had significant transcription difference in the expression of horn bud and skin between horned and polled yaks（P〈0.05）.OLIG2 and ZEB2had highly significant transcription difference in horn bud expression between horned and polled yaks（P 0.01）.We concluded that OLIG2 and FOXL2might be the key candidate genes.ZEB2,TWIST1 and IFNGR2might also involve in theformation of horn.P-cadherin might participate in the development of horn.