副猪嗜血杆菌SC096株荚膜多糖抗猪肺泡巨噬细胞吞噬能力的研究

Role of Capsular Polysaccharide in Haemophilus parasuis SC096 Strain Anti-Porcine Alveolar Macrophage Phagocytosis

为探讨荚膜多糖在副猪嗜血杆菌(Haemophilus parasuis)抗猪肺泡巨噬细胞吞噬中的作用,作者用间接免疫荧光试验和流式细胞术对H.parasuis SC096分离株及其荚膜缺陷型菌株进行了抗巨噬细胞吞噬试验。间接免疫荧光试验显示野生菌株组的H.parasuis大多数存在于巨噬细胞外,而荚膜多糖缺失突变体则主要存在于巨噬细胞内。流式细胞仪检测结果表明,对应的野生型菌株组巨噬细胞总的吞噬率为12.51%,吞入效率为89.61%;而荚膜多糖缺陷型组巨噬细胞总的吞噬率为27.18%,吞入效率为91.06%。加入外源荚膜多糖后,野生型菌株组巨噬细胞总的吞噬率降为2.72%,吞入效率降为32.72%;荚膜多糖缺陷型组巨噬细胞总的吞噬率降为3.59%,吞入效率基本不变,为91.36%。荚膜多糖缺失后H.parasuis抗巨噬细胞吞噬能力降低,表明H.parasuis SC096株荚膜多糖具有抑制巨噬细胞吞噬能力的作用。

The aim of the experiment was to clarify the role of the capsular polysaccharide in antiporcine alveolar macrophage phagocytosis of Haemophilus parasuis.The anti-phagocytosis test was conducted by using indirect immunofluorescence method and flow cytometry with H.parasuis SC096 wild-type strain,and capsular deficient strain.The results showed that the H.parasuis SC096wild-type strains were observed mainly outside the macrophages,while capsular polysaccharide-deficient were observed in macrophages by indirect immunofluorescence experiments.Flow cytometry results showed that the total macrophage phagocytosis rate was 12.51% and swallowed efficiency was 89.61%in the wild-type strain group;the total macrophage phagocytosis rate was 27.18% and swallowed efficiency was 91.06% in the capsular polysaccharide-deficient group.By adding exogenous capsular polysaccharide,total macrophage phagocytosis rate was reduced to 2.72%,swallowed efficiency was reduced to 32.72%in the wild-type strain group;the total macrophage phagocytosis rate dropped to 3.59%,swallowed efficiency was nearly unchanged at 91.36%in the capsular polysaccharide-deficient group.The ability of anti-macrophage phago-cytosis of H.parasuis was reduced through removing capsular polysaccharide,which demonstrated that capsular polysaccharide may inhibit the ability of macrophage phagocytosis.