催产素受体在雌性山羊颈动脉体的分布

Distribution of Oxytocin Receptor in the Carotid Body of Female Goat

研究催产素受体(OTR)在雌性山羊颈动脉体(CB)中是否存在及其分布特点,为催产素(OT)是否对CB起调节作用提供形态学基础。用免疫组织化学SP法对雌性山羊的CB进行OTR免疫组织化学染色,观察CB中OTR的分布特点。结果显示,在雌性山羊CB的实质细胞群和间质组织中均有不同程度的OTR阳性反应产物存在。OTR强阳性产物分布在Ⅰ型细胞、Ⅱ型细胞的细胞质和细胞膜,在Ⅰ型细胞的细胞核呈弱阳性表达,在Ⅱ型细胞的细胞核呈强阳性表达。在间质组织中,血管内皮细胞和神经纤维中有中等阳性反应产物存在,并且实质细胞群和间质组织的OTR相对表达量差异性极显著(P<0.01)。结果表明,OTR主要分布于CB的实质细胞群中,CB具备接受OT调控的条件。因此,催产素可以通过激活CB中的OTR来影响CB的功能活动。

This experiment was conducted to detect the existence of oxytocin receptors（OTR）in the carotid body（CB）of female goats to discuss effects of oxytocin（OT）on the activity of CB.The distribution characteristics of OTR in the carotid body of female goats were studied by using immunohistochemical SP staining.The results showed that,the parenchyma cells and the interstitial tissue were immunolabelled in different degrees.In the typeⅠcells and typeⅡcells of CB,uniformly strong immunostaining was found in the whole cell membrane and cytoplasm,whereas the nucleus of typeⅠ cells were not or weekly immunolabelled,the nucleus of typeⅡcells were strong immunolabelled.In the interstitial tissue,moderate immunoreactivity products distribute in the vascular endothelial cells and nerve fibers.Image analysis documented a significant difference of OTR expression between the parenchyma cells and the interstitial tissue.The OTRs were widely distributed in CB of female goat,suggesting that oxytocin may exert potential effects on carotid body.