基于不同测序技术的生物群落结构及功能菌分析

Analysis of microbial community structure and functional bacteria in a biofilter with different sequencing technologies

分别采用克隆文库和高通量测序技术,解析生物去除铁锰氨滤池内微生物的群落结构和功能菌多样性,并探讨不同测序手段的差异.高通量测序获得15057条有效序列、共32个分类纲,克隆文库测序涵盖9个具有明确分类地位的纲(75条序列),前者能揭示更为丰富的细菌群落结构多样性.功能菌(铁锰氧化细菌和硝化细菌)分析过程中,一些功能菌属在克隆文库中出现,而在高通量测序中未检测到,反之亦然.与单一的测序手段相比,二者相结合能更好地揭示功能细菌的分布特点.

Effects of the technologies of clone library and high throughout sequencing for revealing microbial community structure and functional bacteria in a biofilter which simultaneously removes iron, manganese and ammonia were compared. 15057 sequences with 32 classifications in class level were obtained after the high throughout sequencing process, while there are 9classified groups in clone library. The former revealed more diversity of bacterial community structure. However, in the functional bacteria analysis process, some nitrifiers or iron and manganese bacteria which were detectable in one sequencing analysis process were not appeared in the other process. Comparing with using these two technologies individually in the analysing process, the combined using can compensate for each other and reveal the functional bacteria better.