御寒暖胃膏穴位贴敷对慢性萎缩性胃炎大鼠胃肠激素的影响

Effect of Warm Stomach Cream Acupiontis Stiking Therapy on Gastrointestinal Hormones of Gastric Precancerous Lesions Mouse

目的:研究御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎(CAG)大鼠胃肠激素P物质(substance P,SP)、饥饿素(Ghrelin)、胆囊收缩素(CCK)水平的调节,探讨御寒暖胃膏贴敷胃经穴对CAG大鼠胃黏膜损伤修复的作用机制。方法:大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、药物对照组,采用综合干预方法复制CAG大鼠模型,光镜下观察胃黏膜组织的病理变化,采用酶联免疫分析法(ELISA)测定胃黏膜胃肠激素SP、Ghrelin、CKK的水平。结果:与正常组比较,模型组大鼠的胃黏膜组织病理学检查提示存在腺体的萎缩和一定程度的细胞异型增生,大鼠胃黏膜SP、Ghrelin水平明显降低,差异有统计学意义(P<0.05),CCK水平明显升高,差异有统计学意义(P<0.05);与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜组织的病理损伤得到明显修复,御寒暖胃膏贴敷组SP、Ghrelin水平较模型组明显升高,差异有统计学意义(P<0.05),CCK水平较模型组明显降低,差异有统计学意义(P<0.05)。结论:御寒暖胃膏贴敷胃经穴可以促进CAG大鼠胃黏膜损伤的修复,该作用可能是通过调节胃黏膜SP、Ghrelin、CKK的水平来实现的。

Objective： To study the regulating effect of warm stomach cream acupiontis stiking therapy on the level of gastrointestinal hormones, such as substance P（SP）, Ghrelin and cholecystokinin（CCK）, of mice with Chronic Atrophic Gastritis（CAG）,discuss the mechanism of warm stomach cream acupiontis stiking therapy on repairing gastric mucosal lesion of mice with Chronic Atrophic Gastritis（CAG）. Methods：Mice were randomly divided into normal group,model group,warm stomach cream acupiontis stiking group,drug control group. Duplicating mice model with Chronic Atrophic Gastritis（CAG） in the way of comprehensive intervention,the pathological change of gastric mucosa tissue under the light microscope was measured and the levels of gastrointestinal hormones,such as SP,Ghrelin and CKK of gastric mucosa were observed in the way of ELISA. Results：Comparing with normal group,mice gastric mucosa histopathological examinations in model group reminded that there exist gland atrophy and some degree of cell atypical hyperplasia. The expression levels of SP and Ghrelin in gastric mucosa were dropped obviously（P〈0.05）,and the levels of CCK were obviously increased（P〈0.05）. Comparing with model group,pathological damage of gastric mucosa tissue in warm stomach cream acupiontis stiking group and drug control group was obtained obvious repair. The levels of SP and Ghrelin of mice in warm stomach cream acupiontis stiking group were obviously increased,while the levels of CCK were obviously dropped（P〈0.05 compared with those in model group）. Conclusion：The therapy of the warm stomach cream acupiontis stiking can promote the repair of gastric mucosal lesion. This function may be realized by regulating the expression level of gastric mucosal lesion SP,Ghrelin and CKK.