磷酸酶及张力蛋白同源基因在肺鳞癌中的表达及其临床意义

Expression of phosphatase and tensin homology deleted on chromosometen （PTEN） in squamous-cell lung cancer and its clinical significance

目的 探讨第10号染色体缺失的磷酸酶及张力蛋白同源基因（PTEN）在肺鳞癌（SQCLC）中的表达阳性率及其临床意义.方法 收集煤炭总医院2009年5月至2013年7月住院的50例肺鳞癌组织,其中男43例,女7例,年龄40 ～ 83岁,平均年龄66岁;10例癌旁肺组织,其中男9例,女1例,年龄42 ～ 79岁,平均年龄59岁;13例肺鳞状上皮乳头状瘤石蜡标本作为肺良性病变组织,其中男11例,女2例,年龄34～ 76岁,平均年龄58岁.采用免疫组织化学SP法检测各标本中PTEN的表达,比较SQCLC组、癌旁肺组织及肺良性病变组织PTEN蛋白表达阳性率,分析PTEN表达与患者性别、年龄、吸烟、临床分期、分化程度及淋巴结转移的关系.结果 （1）50例SQCLC中PTEN低表达40例,在癌旁肺组织及良性病变组织中PTEN高表达分别为8和12例;50例肺鳞癌中PTEN表达阳性10例,癌旁肺组织8例,肺良性病变组织12例,差异有统计学意义（x2=23.542,P＜0.01）.（2） PTEN蛋白的表达与患者分化程度、淋巴结转移密切相关（P＜0.05）,而与患者的性别、年龄、吸烟、临床分期无关（P＞0.05）.结论 PTEN蛋白低表达与SQCLC的恶性程度高、发生淋巴结转移密切相关;PTEN蛋白表达缺失可作为评估SQCLC恶性程度的指标之一,是判断预后的独立因素.

Objective To explore the expression of PTEN in squamous-cell lung cancer（SQCLC） and its clinical significance.Methods A total of 50 patients with SQCLC,including 43 males,7 females,aged 40 to 83 （average age 66） years,in Meitan General Hospital from May 2009 to July 2013 were included.Tumor adjacent tissues from 10 patients,including 9 males,1 female,aged 42 to 79 （average age 59）years,and 13 patients with benign pulmonary disease tissues,including 11 males,2 females,aged 34 to 76 （average age 58） years were also included as the controls.The expression of PTEN protein was detected by using immunohistochemistry （S-P）method and compared among SQCLC tissues tumor adjacent tissues and benign disease tissues.The correlations of expression of PTEN protein with gender,age,smoking status,lymph node metastasis,clinical stages and differentiation grades were performed.Results （1） PTEN expression was low in 50 cases SQCLC,while there was a high expression in the tumor adjacent tissues and benign disease tissues.The PTEN protein positive rate of the SQCLC cases （20%,10/50） was significantly lower than that of cases of tumor adjacent tissues and benign disease tissues （8/10,12/13）,x2 =23.542,P 〈0.01.（2） In the SQCLC group,the expression of PTEN protein was significantly related to differentiation grades and lymph node metastasis （P 〈 0.05）,but not gender,age,smoking status,and clinical stages （P 〉 0.05）.Conclusions The lower expression of PTEN in SQCLC was associated with high degree of malignancy and lymph node metastasis.The lost of expression of PTEN may serve as a marker for evaluation of malignancy and an independent factor for prognosis.