不同居群老鸦瓣核型分析

Karyotype Analysis of Different Populations of Tulipa edulis

目的：明确不同居群老鸦瓣核型差异,为老鸦瓣遗传资源保护和人工选育提供依据。方法：采用常规压片法对9个居群老鸦瓣进行核型分析。结果：初步判断9个居群老鸦瓣均为二倍体,其中7个居群染色体基数x=12,染色体数目为2n=2x=24;另有2个居群染色体基数x=24,染色体数目为2n=2x=48。老鸦瓣染色体类型有m、sm、st。染色体比值范围在1.88~2.34之间;不对称系数介于62.99%~71.97%之间;核型有3种类型,包括2A、3A和3B,其中以3B型最多,占67%,且2A型为首次发现。结论：不同居群老鸦瓣在核型上存在较大变异。

Objective： To explore the karyotype difference and to provide basis for genetic resources conservation and breeding of Tulipa edulis. Methods： Karyological analysis on nine Tulipa edulis populations was carried out with conventional pressed slice method. Results： Nine Tulipa edulis populations investigated were diploid. Seven populations of Tulipa edulis were diploid with x = 12,and the chromosome numbers was 2n = 2x = 24. While the others were diploid with x = 24,and the chromosome numbers was 2n = 2x =48. Chromosome types of Tulipa edulis consisted of m,sm and st. The ratio of the longest chromosome to the shortest one ranged from1. 88 to 2. 34. Asymmetry coefficient ranged from 62. 99% to 71. 97%. Karyotype inclucled 2A,3A and 3B,in which 3B type was the most type and 2A type was observed for the first time in Tulipa edulis. Conclusion： The karyological type varies with population of Tulipa edulis.