忍冬木层孔菌醇提物雌激素样作用研究

Estrogenic Activities of Alcohol Extract from Phellinus lonicerinus

目的：研究忍冬木层孔菌醇提物的雌激素样作用,为寻找安全有效的雌激素替代药物提供依据。方法：采用细胞增殖实验评价忍冬木层孔菌醇提物的体外雌激素样作用和抗雌激素作用;检测忍冬木层孔菌提取物对雌性幼鼠、去势雌性大鼠血清中雌二醇（E2）、孕酮（P）含量及成年雌性大鼠子宫雌激素受体α（ERα）、雌激素受体β（ERβ）表达,并考察其对成年雌性大鼠子宫、卵巢及乳腺组织病理学的影响。结果：细胞增殖实验显示,忍冬木层孔菌醇提物在5~50μg/m L时能刺激MCF-7细胞增殖,具有雌激素样作用;在10~100μg/m L时能降低E2诱导的MCF-7细胞增殖,具有抗雌激素作用。体内实验表明300 mg/kg忍冬木层孔菌醇提物能增加雌性幼鼠血清E2含量,促进初生雌性幼鼠子宫发育;忍冬木层孔菌醇提物240 mg/kg可增加去势雌鼠血清E2、P含量,改善因雌激素缺乏而导致的子宫萎缩。120 mg/kg忍冬木层孔菌醇提物可增加正常成年雌性大鼠血清P含量,120、240 mg/kg忍冬木层孔菌醇提物可以增加正常雌性成年大鼠血清E2含量并能调节成年雌性大鼠子宫ERα、ERβ表达。病理检查显示,忍冬木层孔菌醇提物对大鼠子宫、卵巢、乳腺等组织病理学无明显影响。结论：忍冬木层孔菌具有雌激素样作用,无明显不良反应,具有替代雌激素应用的前景。

Objective： To investigate the estrogenic activities of alcohol extract from Phellinus lonicerinus（ AEPL）. Methods： Estrogen and anti-estrogen effects were evaluated by cell proliferation experiment in vitro. Through elevating young rat uterine weight,castrated female rats,and adult female rats uterus index serum estradiol（ E2） and progesterone（ P） were analyzed by enzyme immune methods,and uterine estrogen receptor α（ ERα） and estrogen receptor β（ ERβ） protein expressions were measeured by immunohistochemisty,and investigeted the histopathological of uterus,ovary,and breast of adult female rats. Results： Compared with the control group,AEPL promoted estrogen-sensitive MCF-7 proliferation significantly（ P 0. 05 or P 0. 01） in the doses of 5 ~ 50 μg / m L in vitro experiment;compared with the E2 control group,it also presented anti-estrogenic effect in E2-induced MCF-7 cells at the doses of 10 ~ 100 μg / m L（ P 0. 05 or P 0. 01）. In the animal experiments,AEPL remarkably increased serum E2 content and promoted growth of uterus in primary female mice at the dose of 300 mg / kg; and raised the serum E2 and P content,alleviated uterine atrophy caused by estrogen deficiency in castrated rats at the dose of 240 mg / kg. In adult female rats,AEPL markedly increased the serum P content at the dose of 120 mg / kg,and also markedly increased the serum E2 content at the dose of 120,240 mg / kg,and regulated the protein expressions of ERαand ERβ. AEPL has no effects on histopathological changes of uterus,ovary and mammary gland in rats. Conclusion： AEPL shows estrogenic effects with fewer adverse reaction,which possesses the replacement of estrogen application prospects.