程序冷冻法冷冻精子进行IVF-ET的临床结局分析

The clinical application effect of the programmable freezer method on cryopreservation of human sperm for in vitro fertilization and embryo transfer

目的:探讨程序冷冻法冷冻精子在体外受精-胚胎移植(IVF-ET)中的临床结局及安全性。方法:回顾分析在本中心进行IVF-ET治疗的不孕夫妇的372个周期,分为4个组:用程序冷冻法冷冻精子行常规IVF共76个周期(A组),程序冷冻法冷冻精子行ICSI共92个周期(B组),同期新鲜精液行常规IVF周期97例(C组),新鲜精液行ICSI周期107例(D组),分别比较A组与C组、B组与D组的正常受精率、异常受精率、卵裂率、优质胚胎率、妊娠率、种植率、流产率、多胎率和畸形率。结果:与新鲜精液IVFET组相比较,程序冷冻法冷冻精子常规IVF组及ICSI组的正常受精率、异常受精率、卵裂率、妊娠率、种植率、流产率、多胎率和畸形率等方面差异无统计学意义(P>0.05);但冷冻精子行ICSI组与新鲜精子行ICSI比较,优质胚胎率低且有统计学意义(P<0.05)。结论:用程序冷冻法冷冻精子助孕可以获得与新鲜精子相似的结局,是一种较为安全可靠的精液保存方法,可用于IVF-ET术前精液冷冻保存。

Objectives： To explore the clinical application effect of programmable freezer method on cryopreservation of human sperm for in vitro fertilization and embryo transfer. Methods： A retrospective cohort study was conducted of the 372 cycles of IVF- ET of patients treated in our center. Patients were decided into four groups.Patients in group A underwent 76 cycles of IVF using programmable freezer method on cryopreservation of human sperm. Group B underwent 92 cycles of ICSI using programmable freezer method on cryopreservation of human sperm. Group C underwent 107 cycles of IVF using fresh sperm. Group D underwent cycles of ICSI using fresh sperm. The normal fertilization rate,appearance rate of ≥3PN,clinical pregnancy rate,implantation rate,abortion rate,multiplets rate and deformity rate was respectively compared between group A and group C,group B and group D. Results： No difference was observed among the four groups except for clinical pregnancy rate. Clinical pregnancy rate of group B was significantly lower than that of group D（ P〈0. 05）. Conclusion： Using the programmable freezer method on cryopreservation of human sperm can gain fairly good clinical effect which can be used in IVF-ET preoperative semen cryopreservation.