玉米包衣对种子鉴定过程中PCR扩增的影响

Effects of corn coating on the PCR amplification during seed identification process

目的探讨玉米包衣内含有的杀虫剂、杀菌剂是否影响种子检测过程中的聚合酶链式反应（polymerase chain reaction,PCR）扩增效果及检测结果。方法利用紫外吸收法、凝胶电泳法以及PCR.扩增法对提取的包衣玉米DNA浓度和纯度进行测定,考察PCR扩增效果并对检测结果进行判定。结果不同清洗时间所提取的包衣玉米DNA与未包衣种子提取的DNA质量相比几乎没有差异,浓度和纯度均很好,DNA溶液OD_（260）/OD_（280）比值均在1.7~2.0之间,其DNA质量符合国家种子检测标准要求。玉米包衣对PCR扩增效果及检测结果没有影响。结论包衣玉米样品的种子进行检测时可不作清洗而直接进行DNA提取和PCR扩增,仍可得到准确可靠的结果,从而节约人力和物力并缩短检测时间。

Objective To study the effects of corn coating on amplification effect of polymerase chain reaction（PCR） and test results during seed identification process.Methods The concentration and purity of extracted DNA from coating corn were determined by the method of ultraviolet absorption spectroscopy,gel electrophoresis and PCR amplification.Then the effect of PCR amplification was investigated and the detection results were judged.Results Compared with the quality of DNA extracted from uncoating corn,the quality of DNA extracted from coating corn treated with different cleaning time almost had no difference.Their concentration and purity were both acceptable.The OD_（260）/OD_（280） ratios of DNA solution were between 1.7~2.0.The qualities meet the requirements of national seed testing standards.Corn coating had no effect on PCR amplification and test results.Conclusion The detection of corn samples with seed coating can be performed directly for DNA extraction and PCR amplification without cleaning process,which can still obtain accurate and reliable results,so as to save labor and resources and shorten the testing time.