重性抑郁障碍患者与肿瘤坏死因子ɑ基因启动子区多态性的相关性研究

Association of the Polymorphisms in the Promoter Region of Tumor Necrosis Factor and Interleukin 6 Gene with Major Depressive Disorder

目的探讨肿瘤坏死因子α(tumor necrosis factor,TNF-α)基因启动子区-308G/A、-857C/T位点多态性与重性抑郁障碍症发生的相关性。方法采用聚合酶链限制性片段长度多态性(PCR-RFLP)分析方法检测393例重性抑郁障碍症患者和393名健康对照各个多态性位点的基因型,采用SPSS 13.0进行统计学分析。结果 TNF-α基因启动子-857C/T位点的等位基因和基因型频率分布在正常对照组与重性抑郁障碍症组间存在统计学意义(P<0.05)。其中,男性重性抑郁障碍症组与对照组在-857T的等位基因频率上存在显著差异(OR=0.31,95%CI:0.22-0.44,P<0.01),而女性病例组与对照组在-308A位点的等位基因频率存在显著性差异(OR=0.40,95%CI:0.25-0.65,P<0.01)。结论研究证实TNF-α基因启动子区多态性可能是中国北方汉族重性抑郁障碍患者的风险因素。TNF-α基因启动子区多态性可能与重性抑郁障碍的发病存在关联。

Objective To study the relationship between the- 857C/T and- 308G/A of tumor necrosis factor α gene（ TNF- α） polymorphisms and major depressive disorder. Methods Genomic DNA was gotten from the venous blood leukocytes from 393 patients with major depressive disorder and 393 healthy individuals. Genotype all of the polymorphisms by means of PCR- restriction fragment length poly- morphisms（ PCR- RFLP）. Allele and genotype frequencies were researched by SPSS13. 0 software. Results There were obvious differences in both genotype and allele frequencies of- 857 C/T of TNF- α gene between the major depressive disorder and control groups（ P〈0. 05）. The allele T of-857 C/T in male major depressive disorder group was obviously higher than that in contol group（ OR = 0. 31,95% CI：0. 22- 0. 44,P〈0. 01）. The allele A of- 308 G/A in female major depressive disorder group was obviously higher than that in contol group（ OR = 0. 34,95% CI： 0. 25- 0. 65,P〈0. 01）. Conclusions There is a correlation between-857 C/T of TNF- αgene and major depressive disorder,individuals with T allele of- 857 C/T are susceptible to major depressive disorder,and there is a relationship between- 857 C/T and negative symptom score.