黑龙江地区蒙古族人群HLA-A、B、DRB1高分辨等位基因多态性研究

HLA High Resolution Genotype of Menggu Population in Heilongjiang Region

目的研究中国造血干细胞捐献者资料库黑龙江分库蒙古族捐献者HLA-A、B、DRB1高分辨等位基因频率分布特征。方法采用PCR-测序分型技术(SBT)对67名随机、无血缘关系、健康的中华骨髓库黑龙江分库蒙古族造血干细胞捐献者进行HLA-A、B、DRB1高分辨基因分型,利用计数法和最大似然性原理方法分别计算HLA-A、B、DRB1等位基因频率。结果得到黑龙江地区蒙古族HLA-A、B、DRB1高分辨等位基因频率及分布情况,共观察到80个HLA等位基因,其中A、B、DRB1基因座分别有15、20和14个等位基因有95%的把握认为基因型频率是可靠的(频率>0.022),频率>0.1的等位基因分别为A*24∶02、A*02∶01、A*11∶01、DR*07∶01、DR*09∶01。结论获得黑龙江地区蒙古族人群HLA-A,B,DRB1高分辨等位基因频率数据,其具有独特的基因频率分布特征,但与其他地区蒙古族最为相近。

Objective To research the distribution features of HLA- A,B and DRB1 high resolution alleles in the Menggu population from Heilongjiang region. Methods PCR- SBT methods was applied for HLA- A,B,DRB1 high resolution genotype of 67 unrelated and healthy donors in the Menggu population from Heilongjiang region,and gene frequencies were calculated by counting and maximum likelihood method. Results A Total of 80 HLA alleles were observed and the most frequent alleles（ 〉0. 1） were A＊ 24 ∶ 02,A＊ 02 ∶ 01,A＊ 11 ∶ 01,DR＊ 07 ∶ 01 and DR ＊ 09 ∶ 01.Conclusions Get the distribution features of HLA high resolution allele in the Menggu population from Heilongjiang region,Distribution of alleles have their own distribution,but close to Menggu population from other region.