抗白灵霜在黑素合成中对TYR和MITF的调节作用

The regulative effect of Kang Bai Ling cream on TYR and MITF in melanogenesis

目的:明确抗白灵霜(KBL)在黑素合成中对TYR和MITF的调节作用。方法:体外培养小鼠B16黑素瘤细胞,分为空白对照组和不同浓度KBL(5 mg/mL,10 mg/mL和15 mg/mL)用NaOH溶解法检测黑素合成量,Real time-PCR检测TYR和MITF基因mRNA表达,Western Blot检测KBL组TYR和MITF蛋白的表达。结果:各浓度KBL组黑素合成量及TYR和MITF基因的mRNA和蛋白表达的水平均高于空白对照组,且呈浓度依赖性。结论:KBL可能通过上调MITF基因的表达,激活参与黑素合成的TYR以促黑素合成。

Objective：To determine the regulative effect of Kang Bai Ling（KBL） creams on TYR andMITF in melanogenesis.Methods：The cultured B-16 murine melanoma cells were divided into blank groupand KBL groups with different concentration（5 mg/ mL,10 mg/ mL and 15 mg/ mL）.The amount of melaninwas detected by NaOH dissolved method.The expression of TYR and MITF mRNA and protein was detected byreal time-PCR and Western blot respectively.Results：The amount of melanin,the expressions of TYR andMITF mRNA and protein in KBL groups were higher than those in blank group.The effects were significant ina concentration dependent manner.Conclusion：KBL can promote melanogenesis through up-regulating theexpression of MITF genes and activating TYR.