不同来源红檵木材料的RAPD分析及分类学探讨

RAPD Analysis and Taxology Discussion on Loropetalum chinense var.rubrum Materials from Different Fountainhead Areas

以 2个生态型檵木材料为对照 ,对在各原产地收集到的 14个红檵木材料进行 5 5个随机引物的RAPD反应 ,共产生 6 32条稳定的扩增带 ,其中 42 7条具有多态性。按扩增带的有无列出二元数据矩阵 ,利用NTSYS pc计算Nei氏相似系数及遗传距离 ,利用SAS软件进行主成分分析。结果表明 :红檵木是檵木的变种 ,供试的 14个红檵木材料分属 4类 ,其突变途径可能是一个复杂的过程 ,存在着檵木不同生态型间、檵木与红檵木之间 ,红檵木之间的相互作用。

Contrasted with 2 different Loropetalum chinense ecotypes,fourteen Loropetalum chinense var.rubrum materials representing almost all the types could be were analyzed by RAPD in this paper.Fifty-five random primers produced 632 steady amplified bands in total,among them 427 bands were polymorphic.Strong and clear bands were scored for their presence or absence in a binary data matrix.Nei's similarity coefficients and genetic distances were calculated by NTSYS-pc software,and principal components analysis was done by SAS software.The result demonstrated that L.chinense var.rubrum is a mutant of L.chinense on molecular level and 14 L. chinense var.rubrum materials were classified into 4 groups.The mutant pathway of 4 groups is possibly a complicated process,including interactions between different ecotypes L.chinense,interactions between L. chinense and L.chinense var.rubrum,and ineractions between different L.chinense var.rubrum.