电场诱导原生质体融合获得柑橘属间四倍体体细胞杂种

Production of Citrus Intergeneric Tetraploid Somatic Hybrids Plant from Electricity-mediated Protoplast Fusion

采用电融合方法诱导澳洲指橘 (MicrocitruspapuanaSwingle)悬浮系原生质体与柑 (CitrusreticulataBlanco)叶肉原生质体融合 ,得到了再生植株。染色体检查表明再生植株为四倍体 ( 2n =4x =36 ) ,6个 10 mer碱基随机引物PCR扩增结果表明 ,尽管再生植株带型表现多样 ,但仍然可以证明为体细胞杂种。对再生植株田间生长异常的现象进行了讨论。

Ponkan (Citrus reticulata Blanco), which is very seedy, is one of the most widely grown mandarin cultivars in China for its good quality. Mitochondrial genome of Microcitrus papuana is related to seedlessness. But it is of difficulty to transfer the cytoplasmic genome of the latter. The present research involves electrofusing embryonic protoplasts of M. papuana and leaf-derived protoplasts of Ponkan, from which plants were obtained. Chromosome detection showed that the plants were tetraploids with 36 chromosomes. Amplification with six 10-mer arbitrary primers identified the plant as true somatic hybrids though complex band profiles of the regenerated plant were observed. The somatic hybrid plant holds great potential for breeding seedless varieties of Citrus. In addition, abnormal growth of the plant in field is discussed herein.