4种中草药乙醇提取物体外抗氧化及美白活性研究

Test for antioxidation and whitening activity of ethanol extract from four kinds of Chinese herbal medicine in vitro

采用乙醇浸提法从干燥的芦荟叶、白芷、怀山药和黄芩4种中草药中提取分离得到其乙醇提取物。应用自由基清除活性和总抗氧化活性检测方法评估这4种中草药乙醇提取物的抗氧化活性,应用酪氨酸酶活性抑制实验、黑色素B16细胞增殖抑制和黑色素生成抑制实验对其进行美白活性评估。结果表明:4种中草药的乙醇提取物虽然具有一定的抗氧化活性、清除自由基能力、抑制酪氨酸酶活性、抑制黑色素B16细胞增殖和黑色素形成作用,但与市售护肤品常用的抗氧化美白功效成分BHT(2,6-二叔丁基-4-甲基苯酚)相比,其活性水平偏低,需要进一步分离纯化,富集活性成分,提高抗氧化美白活性。

Extracts,with ethanol as solvent,from 4 kinds of Chinese herbal medicine（ dried aloe leaves,Angelica dahurica,Huaiqing yam and Radix scutellariae） were assessed for their antioxidation activity by free radical scavenging activity detection method and total antioxidation activity detection method. Meanwhile,their human skin whitening activity was assessed by tyrosinase inhibition test,B16 melanoma cells proliferation inhibition test and melanin formation inhibition test. The results showed that although the aforesaid four kinds of Chinese herbal medicine extract display certain antioxidation and free radical scavenging activity as well as tyrosinase inhibition activity,B16 melanoma cells proliferation inhibition and melanin formation inhibition capacity; however,as comparing with BHT（ 2,6- di- tert- butyl- 4- methylphenol） that containing in conventional skin care cosmetic products from the market,their antioxidation and whitening effect are lower. So it is necessary to separate and purify the ethanol extracts for enriching the active ingredients,as well as to improve their antioxidation and whitening activity.