后肢接振对家兔脑和骨骼肌组织中calpain mRNA表达的影响

Effects of expression of calpain mRNA in rabbits exposed to vibration by hind legs

目的探讨后肢接触振动（简称接振）对家兔脑和骨骼肌组织中calpainmRNA表达的影响。方法依据4h等能量频率计权加速度有效值【ahw（4）】将32只新西兰大白兔随机分为低强度（4．33m／s^2）、中强度（8．67m／s^2）、高强度（17．34m／s^2）组和对照组；后肢接振45d后，取家兔脑和骨骼肌组织，实时荧光定量聚合酶链式反应（RT-qPCR）检测其calpain-1和calpain-2mRNA的表达情况。结果低、中、高强度组家兔脑组织中calpain-1mRNA的相对含量分别为8．35±3．75、9．64±4．54、15．10±5．26，calpain-2mRNA的相对含量分别为7．34±4．97、8．50±5．66、8．16±5．59，明显高于对照组（1．10±0．29、0．56±0．43），差异均有统计学意义（P〈0．01）；与对照组（0．98±0．59）比较，低、中、高强度组家兔骨骼肌组织中calpain-1mRNA的相对含量（4．36±2．05、7．37±4．06、12．46±6．21）明显升高，差异均有统计学意义（P〈0．05）；各强度组与对照组家兔骨骼肌组织中calpain-2mRNA的表达差异无统计学意义（P〉0．05）。结论后肢接振能够上调家兔脑组织中calpain-1和calpain-2mRNA以及骨骼肌组织calpai-1 mRNA的表达。

Objective To study the effects of expression of calpain mRNA in rabbits exposed to vibration by hind legs. Methods 32 New Zealand rabbits were randomly divided into a control group and 3 experimental groups according to d-hour energy-equivalent frequency-weighted acceleration[ahw（4）] ：low （4.33 m/s2）, moderate （8.67 m/s2） and high （17.34 m/s^2） intensity group to accepted the vibration by hind legs. 45 ds later, brain and skeletal muscle tissue of rabbits were taken to detect the expression of calpain-1 and calpain-2 mRNA by RT- qPCR technique. Results The relative content of calpain-1 mRNA in the brain tissues in rabbits of low, medium and high intensity group were 8.35±3.75,9.64±4.54,5.10±5.26. While the relative content of calpain-2 mRNA in the brain tissues in rabbits of low, medium and high intensity group were 7.34±4.97,8.50±5.66,8.16±5.59. Compared with the control group（ 1.10±0.29,0.56±0.43 ）, the expression of ealpain-1 and calpain-2 mRNA of the intensity groups showed an significantly increasing trend（P〈0.01 ）. In skeletal muscle tissue, the relative content of calpain-1 mRNA were 4.36±2.05,7.37±4.06,12.46±6.2LCompared with the control group （0.98±0.59）, the expression of ealpain-1 mRNA of experiment groups were significantly higher （P〈0.05） .The expression of calpain-2 mRNA of the intensity groups had no significantly difference with the control group （P〉0.05）. Conclusion The expression of ealpain-1 and calpain-2 mRNA can be promoted by the vibration by the hind legs.