酸碱度对玉米秸秆酶解液光合生物产氢动力学的影响

Effect of pH values on the biohydrogen production from the enzymolysis liquid of the corn stalks via the photosynthetic bacteria

主要研究p H值对玉米秸秆酶解液光合生物产氢动力学的影响,以生物量干重为指标研究p H值对光合产氢细菌生长的影响,以产氢速率和产氢量为指标研究p H值对光合细菌产氢的影响。利用Logistic模型和MMF模型分别对光合细菌生长和玉米秸秆酶解液光合生物产氢进行了回归模拟,提出了p H值对光合细菌生长动力学和玉米秸秆酶解液产氢动力学的影响规律。结果表明：p H值对光合细菌生长及产氢过程都有显著影响,随碱性增强,光合细菌生物量逐渐增大,当p H值为8时,光合细菌生物量达到最大,为0.543 7 g/L,最佳接种时间为84~96 h;光合细菌产氢量随p H值增加同样呈现先递增再递减的趋势,当p H值为6时,产氢速率和产氢量达到最大,分别为29.72 m L/（L·h）、168.96 m L。

This paper is aimed to explore the effect of pH value on the hydrogen-producing kinetics by photosynthetic bacteria from the enzymolysis liquid from the cornstalks.Taking the dry weight of biomass as assessment indicators,we have studied the effect of the different pH values on the growth of photosynthetic bacteria.In proceeding with our research,we have done the regression simulation for the growth of photosynthetic bacteria and photosynthetic bacteria hydrogen production from the enzymolysis liquid of corn stalks by using the logistic model and MMF model.As a result,we have found the effect of the different pH values on the features of hydrogen production kinetics and the growth conditions of photosynthetic bacteria,which implies that different pH values tend to affect the growth kinetics of photosynthetic bacteria differently whereas the hydrogen production kinetics of corn stalks enzymolysis liquid significantly.We have also found that pH value has a significant impact on the growth of the photosynthetic bacteria and hydrogen production process.For example,with the increase of pH,the photosynthetic bacterial biomass tends to increase in a proportional manner.When the pH value is 8,the maximum of the photosynthetic bacterial biomass turns to be0.543 7 g/L with the best time for inoculation being 84-96 h.What is more,the hydrogen production of photosynthetic bacteria tends to increase first and then decrease with the growth of pH value.When the pH value becomes 6,it has been found that the rate and capacity of the hydrogen production may reach its maximum degree,29.72 mL/（L·h） and 168.96 mL respectively.The results of our experiment can help to provide a scientific reference for further improvement of the enzymatic saccharification of the corn stalks on the photosynthetic hydrogen production theoretically and technically.