地西泮及去甲地西泮在大鼠体内的死后再分布研究

Study on the postmortem redistribution of diazepam and nordiazepam in poisoned rats

目的观察地西泮及其主要代谢物去甲地西泮在大鼠体内的死后再分布特点。方法大鼠24只,随机分成8组,以安定90 mg/kg灌胃,2 h后脱颈椎处死,左侧卧位,分别置于室温(10℃)条件下,于死后不同时间(0h、2h、4h、8h、12h、24h、48h、96h)取心、肝、脾、肺、肾、大脑、肌肉,固相萃取、高效液相色谱法检测其中地西泮及其主要代谢物去甲地西泮的含量。结果地西泮及其主要代谢物去甲地西泮在染毒处死大鼠内各时间点、各脏器都有分布并呈现出一定的规律性。结论地西泮及其主要代谢物去甲地西泮在染毒处死大鼠内可发生死后再分布。

Objective To explore the postmortem redistribution of diazepam and nordiazepam in acute poisoned rats. Methods The 24 rats in experimental group were randomly divided into eight groups ,and given intragastric ministration of diazepam with a dose of 90 mg/kg, 2h later, they are executed by hypoxia method and then they are preserved at temperature of 10~C. Cardiac, liver, spleen, lung, kidney, brain and muscle are taken out to detect diazepam and nordiazepam at different times after death （Oh, 2h, 4h, 8h, 12h, 24h, 48h, 96h ）, which were then extracted with a solid phase extraction （SPE） and determined by HPLC-MS-MS. Results The distribution regularities of diazepam and nordiazepam of different visceras in acute poisoned rats would change by the time. Conclusion The diazepam and nordiazepam in acute poisoned rats could have the postmortem redistribution.