AChE、BChE、PON-1和FOS mRNA在甲拌磷急性中毒致死大鼠脏器中的表达

The expression difference of AChE, BChE, PON-1 and FOS mRNA in rats died of acute phorate poisoning

目的通过对甲拌磷急性中毒致死大鼠脏器中乙酰胆碱(ACh E)、丁酰胆碱(BCh E)、对氧磷酶1(PON-1)和原癌基因蛋白(FOS)m RNA的表达研究,筛选出表达变化有统计学意义的脏器和中毒相关基因,探讨其在死亡原因推断中的价值。方法健康成年雌性SD大鼠,随机分为空白对照组和急性中毒死亡组,分别取心、肝、肺、脑和肠,提取总RNA,加入目的基因ACh E、BCh E、PON-1、FOS和内标基因β-肌动蛋白(β-actin)引物逆转录成c DNA后,利用实时荧光定量PCR技术(RT-PCR)检测各组c DNA的相对表达量的变化,并对各组结果进行统计学分析。结果肝脏中ACh E、BCh E和FOS m RNA的表达均上调(P<0.05),ACh E m RNA在肺和脑中的表达均下调(P<0.05)。结论肝脏和ACh E分别可以作为甲拌磷急性中毒致死的靶器官和基因表达标志,能为今后急性甲拌磷中毒的临床诊断与死亡推断提供依据。

Objective To investigate mRNA expression of the related genes （AChE,BChE,PON-1 and FOS） in SD rats died of acute phorate poisoning, and screen the significant organs and genes in the detection of death. Methods 12 grown-up female SD rats were divided into the acute poisoning group and the control group. Organ samples（ heart, liver, lung, brain, small intestine） were collected respectively, and total RNA were isolated to be reversely transcribed to eDNA with gene primers of ACHE, BChE, PON-1, FOS and interior label β-actin. The Real Time PCR was used to detect the comparative expression differences of them and the data of all the groups were analyzed statistically. Results In liver, the expression of ACHE, BChE and FOS mRNA increased （P〈0.05）, while the expression of AChE mRNA decreased in lung and brain （P〈0.05）. Conclusion Liver and AChE could be served as the target organ and the gene expression mark for death by acute phorate poisoning respectively, which could also be set as the reference of the diagnose and death detection from acute phorate poisoning.