摘要
目的研究碘离子(I-)对血管内皮细胞(VEC)迁移及细胞分裂周期蛋白42(Cdc42)表达的影响,探讨细胞迁移与Budd-Chiari综合征隔膜形成机制。方法将体外培养的VEC分为空白对照组和不同I-浓度实验组,用Transwell法检测各组细胞迁移数、I-与Cdc42抑制剂ML141作用后VEC迁移情况。采用Western blot技术检测不同I-浓度培养环境中Cdc42表达。结果一定浓度的I-可以促进VEC迁移(P<0.05);ML141可抑制VEC迁移;100μg/L I-组、300μg/L I-组、500μg/L I-组VEC Cdc42相对表达量高于其它各组(P<0.05)。结论碘能促进VEC迁移,该效应可能与调整Cdc42表达有关。
Objective To investigate the effect of iodineion (I^-) on the migrationof vascular endothelial cell (VEC) and on the expression of cell division cycle 42( Cde42), and to discuss the relationship between cell migration and the membranous formation in Budd-Chiari syndrome. Methods VECs that were cultured in vitro were divided into the blank control group and four experimental groups of different I^- concentration (100 μg/L I^-, 300 μg/L I^-, 500 μg/L I-and 1000 μg/L I^-respectively).The number of cell migration and the migration situation of VEC after the interaction of I^- with Cdc42 inhibitor ( ML141 ) in each group were assessed by Transwell chamber motility assay. Using Western blot method the expressions of Cdc42 in different Iμ-concentration culture environment were determined. Results Certain concentration of I^-could stimulate the migration of VEC (P〈0.05). ML141 could inhibit the migration of VEC; the relative expressions of VEC Cde42 of 100 μg/L I^- ,300 μg/L I^- and 500 μg/L I^-groups were significantly higher than those of other groups (P〈0.05). Conclusion Iodine can promote VEC migration, and this effect may be related to the regulation of Cdc42 expression.
出处
《介入放射学杂志》
CSCD
北大核心
2016年第6期524-528,共5页
Journal of Interventional Radiology
基金
江苏省科技创新与成果转化专项基金(BL2012021)