碘离子调控血管内皮细胞迁移机制研究

The role of iodine ions in regulating the migration of vascular endothelial cells

目的研究碘离子(I-)对血管内皮细胞(VEC)迁移及细胞分裂周期蛋白42(Cdc42)表达的影响,探讨细胞迁移与Budd-Chiari综合征隔膜形成机制。方法将体外培养的VEC分为空白对照组和不同I-浓度实验组,用Transwell法检测各组细胞迁移数、I-与Cdc42抑制剂ML141作用后VEC迁移情况。采用Western blot技术检测不同I-浓度培养环境中Cdc42表达。结果一定浓度的I-可以促进VEC迁移(P<0.05);ML141可抑制VEC迁移;100μg/L I-组、300μg/L I-组、500μg/L I-组VEC Cdc42相对表达量高于其它各组(P<0.05)。结论碘能促进VEC迁移,该效应可能与调整Cdc42表达有关。

Objective To investigate the effect of iodineion （I^-） on the migrationof vascular endothelial cell （VEC） and on the expression of cell division cycle 42（ Cde42）, and to discuss the relationship between cell migration and the membranous formation in Budd-Chiari syndrome. Methods VECs that were cultured in vitro were divided into the blank control group and four experimental groups of different I^- concentration （100 μg/L I^-, 300 μg/L I^-, 500 μg/L I-and 1000 μg/L I^-respectively）.The number of cell migration and the migration situation of VEC after the interaction of I^- with Cdc42 inhibitor （ ML141 ） in each group were assessed by Transwell chamber motility assay. Using Western blot method the expressions of Cdc42 in different Iμ-concentration culture environment were determined. Results Certain concentration of I^-could stimulate the migration of VEC （P〈0.05）. ML141 could inhibit the migration of VEC; the relative expressions of VEC Cde42 of 100 μg/L I^- ,300 μg/L I^- and 500 μg/L I^-groups were significantly higher than those of other groups （P〈0.05）. Conclusion Iodine can promote VEC migration, and this effect may be related to the regulation of Cdc42 expression.