六味地黄丸对甘油诱导的急性肾损伤大鼠肾小管间质的影响

Effects of Liuwei Dihuang Pills on Renel Tubular in Rats with Acute Kidney Injury

目的观察六味地黄丸对甘油诱导的急性肾损伤大鼠肾小管间质的影响。方法将36只Wistar大鼠按随机数字表分为正常组、模型组、六味地黄丸组。模型组与六味地黄丸组大鼠用50%甘油生理盐水肌注,建立急性肾损伤大鼠模型。正常组大鼠使用等剂量生理盐水肌肉注射。造模结束后,模型组及正常组给予生理盐水灌胃,六味地黄丸组给予六味地黄丸混悬液灌胃。治疗3d后检测各组血清BUN、Scr,尿NAG、β2-MG及尿渗量,并进行肾脏组织形态学观察。结果六味地黄丸组大鼠血清BUN、Scr、尿NAG酶、β2-MG较模型组明显下降,而尿渗量升高,肾脏病理改变好转,差异有统计学意义(P<0.05)。结论六味地黄丸能减轻急性肾损伤大鼠肾脏病理改变,能降低BUN、Scr及尿NAG酶水平,具有肾脏保护作用。

Objective To study the preventive effects of Liuwei Dihuang pills on renel tubular in rats with acute kidney injury. Methods36 Wistar rats were randomly divided into normal group,model group and Liuwei Dihuang pills treatment group. Rat models of acute kidney injury were established with injecting glycerol in dose of 10 m L / kg. Liuwei Dihuang pills was administered intravenously into rats of treatment group in 1. 5g / kg / d for 3d. Levels of blood urea nitrogen（ BUN）,serum creatinine（ Scr）,urine N- acetyl- D- glucosaminidase（ NAG）,urine β2- microglobulin（ β2- MG） and urinary osmolalities in the 3 groups were assayed at the late of medication. Renal histological examination was also performed. Results Compared with model group,the levels of BUN and Scr,NAG,β2- MG in Liuwei Dihuang pills treatment group were significantly lower,but urine osmolality remarkably increased in comparison with model group. Liuwei Dihuang pills also lessened the histological changes in treatment group. Conclusion Liuwei Dihuang pills have some protective effects on rats with acute kidney disease.