摘要
目的探讨钙依赖的氯离子通道Anoctamin5在骨骼肌发育过程中的可能作用。方法采用RT-PCR检测Anoctamin5 m RNA在小鼠骨骼肌发育的不同时期的表达变化,同时,采用免疫荧光,Western blot的方法检测Anoctamin 5蛋白在小鼠骨骼肌发育不同时期的表达变化。结果 Anoctamin5m RNA与蛋白在1 d至6月的小鼠骨骼肌中均有表达,但随着年龄的增长,Anoctamin5 m RNA与蛋白的表达呈逐渐降低的趋势。结论 Anoctamin5在骨骼肌的发育过程中发挥着重要的作用,也可能参与骨骼肌的分化、增殖及损伤修复,这也与Anoctamin5突变的病人出现症状较晚相一致。
Objective To study the possible role of the calcium dependent Anoctamin5 chloride ion channels in the process of skeletal muscle development. Methods RT-PCR was used to detect Anoctamin5 mRNA expression in skeletal muscle of different development periods in mice, and immunofluorescence and Western blot methods was used to detect Anoctamin 5 protein expression in different development periods of mice skeletal muscle. Results Anoctamin5 mRNA and protein expression in skeletal muscle in mice from 1 day to 6 months, but with the growth of the age, Anoctamin5 mRNA and protein expression underwent a trend of gradual reduction. Conclusion Anoctamin5 plays an important role in the development process of skeletal muscle in mice, which may participate in differentiation, proliferation, injury and repair of skeletal muscle. Our foundings are also with consistent with the patient developed symptoms later caused by Anoctamin5 mutations.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2016年第3期318-321,共4页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金(31200858)
新乡医学院科研培育基金(2014QN119)