鼠神经生长因子治疗胫骨骨不连效果的实验研究

The researchon rat nerve growth factor in the treatment of tibial fracture nonunion

目的研究鼠神经生长因子（NGF）对白兔胫骨骨不连的治疗效果,为NGF在骨不连的临床运用提供实验基础。方法选择健康成年雄性新西兰大白兔40只,随机各选20只分为实验组和对照组,分别于右胫骨粗隆下3～5 cm处造成横行骨折骨不连模型,实验组在骨折处每周注射NGF一次,连续12周;对照组在骨折处每周注射生理盐水一次,连续12周。试验期间两组均同时接受X线和组织学检查以观察骨痂生长和骨折愈合情况。结果实验组动物骨痂出现时间为（2.0±0.5）周,骨折线模糊时间为（8.2±1.2）周,骨折愈合时间为（12±1.7）周;对照组骨痂出现时间为8周以上,未出现骨折线模糊和骨折愈合。实验组骨折愈合情况远好于对照组,差异具有统计学意义（P〈0.05）。结论 NGF有较强的促进骨不连骨痂生长和愈合作用,NGF在骨不连的临床治疗中有良好的运用前景。

Objective To study therapeutic effect of rat nerve growth factor （NGF） on rabbit tibial nonunion treatment and provide a reference for its clinical application. Method 40 healthy adult male New Zealand white rabbits underwent establishment of transverse fracture nonunion model about 3-5 cm under the right tibial tuberosity, and were divided randomly and equally into the experimental group and the control group： the experimental group received injection of NGF at the fracture site once a week for 12 weeks; the control group received injection of normal saline at the fracture site once a week for 12 weeks. Both groups received X-ray and histological observation in the same time to observe callus growth and bone healing during the experiment. Results In the experimental group, callus appeared in （2.0±0.5） weeks, the fracture line became blurred in （8.2±1.2） weeks, and bone healed in （12～1.7） weeks; in the control group, callus appeared in more than 8 weeks, no fracture line became blurred or bone healed. Fracture healing in the experimental group was much better than that in the control group. According to the statistics processing,the difference had statistical significance（P〈0.05）. Conclusion NGF has a strong promotion of bone nonunion callus growth and healing, and its broad application prospect in the clinical treatment of nonunion might be viewed.