益气活血方对大鼠慢性脊髓压迫后受损脊髓组织形态学及其PGE_2、PLA_2含量的影响

Effects of QNPAB on Morphological Changes and the Contents of PGE2 and PLA2 in Damaged Rats′ Spinal cord Tissue of the Chronic Spinal Oppression Model

目的观察不同浓度的益气活血方对大鼠慢性脊髓压迫后受损脊髓组织的形态学及其前列腺素E_2(PGE_2)、磷脂酶A_2(PLA_2)含量的影响。方法 60只SD大鼠,随机选取10只作为假手术组,仅暴露椎板,未造成脊髓压迫,以0.9%氯化钠注射液进行灌胃;其余的SD大鼠采用自体第7颈椎棘突椎间移植法制造大鼠脊髓压迫模型,术后1 d根据脊髓功能的评分将其分为,A组造模后,以不同浓度的益气活血方进行灌胃;B组造模后,以颈复康进行灌胃;C组造模后,以0.9%氯化钠注射液进行灌胃。灌胃结束,处死动物并取其受损脊髓组织做以下处理:1)经HE染色后观察受损脊髓组织的形态学改变;2)采用ELISA方法测其PGE_2、PLA_2的含量。运用统计学手段对实验结果进行分析,基于上述结果综合评估益气活血方和颈复康对炎性介质的抑制功效。结果受损脊髓组织形态学的观察结果显示;中药高、中、低浓度组以及颈复康组的受损脊髓组织内均可见胶质瘢痕和空洞形成,且空洞总面积均明显小于NS组。通过检测大鼠受损脊髓组织中的炎症因子发现,中药中浓度、中药高浓度及颈复康均能显著降低受损脊髓组织内PGE_2的含量(P<0.05),各浓度的益气活血方及颈复康均能显著减低受损脊髓组织内PLA_2的含量(P<0.05)。结论在大鼠慢性脊髓压迫模型中,益气活血方能有效抑制受损脊髓组织的炎症反应,并促进其脊髓功能的恢复。

Objective： To evaluate the potential effects of different concentrations of PNQAB（the prescription for nourishing qi and activating blood） on morphological changes and contents of PGE_2,PLA_2 in the damaged rats ′spinal cord tissue. Methods： Ten SD-Rats were randomly chosen as the sham-operated group from the sixty subjects,fed with the normal saline within explosion of the vertebral lamina and free from oppression of the spinal cord;the remaining were applied to establish rat′s spinal oppression model by grafting the self-7th cervical vertebrae spinous process at the part of C4~5 or C5~6 intervertebral space,then divided into three groups based on the assessment results of the spinal cord function at the 1st day postoperatively,fed with the PNQAB under different concentrations（Group A）,Jinfukang（Group B） and Normal saline（Group C）,respectively. All the animals were sacrificed by the end of the gastric perfusion and the damaged tissue of spinal cord was removed for the examination of morphological changes（after HE staining） and the determination of contents of PGE_2 and PLA_2（tested with ELISA method） of the specific tissue in rats′ spinal cord. Results： The results of HE staining showed that glial scars and cavitation were sporadically formatted in Group A and Group B,but existed widely in Group C,and the total areas of cavitation in Group A and Group B were obviously less than Group C. Furthermore,groups fed with the PNQAB under high/medium concentration and Jinfukang could notably decreased the content of PGE_2（P〈0.05）,while all the groups fed with the PNQAB and Jinfukang had a positive effects on the inhibition of PLA_2 production in the tissues of the rats′ damaged spinal cord significantly（P〈0.05）,compared with those fed with normal saline. Conclusions： The PNQAB effectively inhibits inflammatory response in tissues during rat′s recovery of chronic spinal oppression model,and promotes the recovery of spinal cord function. It is an effective treatment method,worthy of further promotion.