益气活血保心汤对急性心肌梗死大鼠的保护作用

Effect of Yiqi Huoxue Baoxin Decoction on Rats with Acute Myocardial Infarct

目的探讨益气活血保心汤对急性心肌梗死大鼠的保护作用。方法 SD大鼠50只随机分为假手术组、模型组、益气活血保心汤低剂量组、益气活血保心汤高剂量组和复方丹参滴丸组。采用结扎冠状动脉左前降支的方法建立急性心肌梗死大鼠模型,造模成功后于不同时间点分别比较大鼠心电图的变化情况,采用TTC染色法记录心肌梗死面积,并测乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)、肿瘤坏死因子-α(TNF-α)、C反应蛋白(CRP)的血清浓度。结果实验过程中大鼠先后死亡共计5只,死亡原因为恶性心律失常,其中模型组、益气活血低剂量组各2只,益气活血低剂量组1只,符合急性心肌梗死死亡率高的特点。与结扎前比较,在结扎即刻模型组、益气活血保心汤高剂量组、益气活血保心汤低剂量组以及复方丹参滴丸组J点抬高均≥0.1 mV,提示造模成功。与假手术组比较,模型组结扎24 h后ΔJ明显抬高(P<0.01);与模型组比较,药物组ΔJ明显降低(P<0.01或P<0.05),其中益气活血保心汤高剂量组、复方丹参滴丸组最为明显(P<0.01)。与假手术组比较,模型组LDH、CK-MB水平显著升高(P<0.01);与模型组比较,益气活血保心汤高剂量组与复方丹参滴丸组均显著降低LDH、CK-MB的血清浓度(P<0.01);益气活血保心汤低剂量组可降低CK-MB浓度(P<0.05),但LDH浓度无明显差异(P>0.05)。与假手术组比较,模型组TNF-α、CRP水平显著升高(P<0.01);与模型组比较,各治疗组均显著降低血清TNF-α、CRP的浓度(P<0.01或P<0.05)。与假手术组比较,模型组心梗面积明显增大(P<0.01);与模型组比较,各给药组心梗面积明显减小(P<0.01或P<0.05)。益气活血保心汤高剂量组与复方丹参滴丸组相比较,差异无统计学意义(P>0.05)。结论急性心肌梗死大鼠心电图J点出现异常变化,血清心肌酶学明显升高。益气活血保心汤能够有效抑制急性心肌梗死对大鼠造成的损伤,其作用机制可能与其抑制炎症反应,降低TNF-α、CRP水平有关。

Objective： To discuss the effects of Yiqi huoxue baoxin Decoction on acute myocardial infarction（AMI） in rats. Methods： 50 healthy SD rats were randomly divided into sham operation group,the model group,lower dosage group of Yiqi huoxue baoxin Decoction,higher dosage group of Yiqi huoxue baoxin Decoction and the Compound Danshen Pill group. Acute myocardial infarct（AMI） model was established by coronary artery ligation. After successful modeling,the electrocardiogram ECG was recorded and the Myocardial infarct area was detected by TTC stain. Blood was collected to determine the contents of lactate dehydrogenase（LDH）,creatine kinase isoenzymes（CK-MB）,tumor necrosis factor-α（TNF-α）,and C-reactive protein（CRP）. Results： In comparison with the model group,the treatment groups can improve the abnormal changes of ECG induced by acute myocardial infarct,reduce myocardial infarction size and lower the level of myocardial enzyme,CRP and TNF-α.Conclusion： J wave of rats with acute myocardium infarct drifts evidently,and myocardial enzyme was significantly higher. In this experiment,Yiqi huoxue baoxin Decoction plays an effective role in alleviating the injuries in AMI rats. The mechanisms may be through decreasing anti-inflammation reaction and reducing contents of TNF-α and CRP.