胶体金在不同培养温度鼠疫FI抗原检测中遇到的问题

Explore the problems of colloidal gold paper chromatography in plague FI antigen detection

目的对比胶体金纸上色谱试验(RGICA)、反向血凝试验(RIHA)及细菌检验对于28℃与37℃鼠疫菌的敏感性及特异性。方法 28℃、37℃鼠疫菌培养24 h制备菌液与28℃、37℃的鼠疫菌感染脏器取材制成的菌悬液分别行反向血凝试验,细菌检验及胶体金纸上色谱试验。结果 37℃鼠疫菌悬液及37℃鼠疫菌感染动物制备菌悬液,胶体金、反向血凝试验及细菌检验均敏感,28℃及28℃鼠疫菌感染动物取材制备的菌悬液反向血凝试验、细菌检验敏感,胶体金不敏感。结论在现场应用与鼠疫监测中反向血凝试验因其具有简便、快速、敏感已被列入标准,28℃鼠疫菌胶体金检测敏感性差,检测FI抗原缺乏的鼠疫菌株易漏诊,只能作为辅助诊断。

Objective Compared the specificity and sensitivity of colloidal gold paper chromatography （RGICA） , reverse indirect hemagglutination assay （RIHA） and bacteria inspection for Yersinia pestis which were cultured at 28 ℃ and 37 ℃, respectively.Methods Yersinia pestis were cultivated 24 h at 28 ℃ and 37 ℃ to prepare with bacteria liquid and infected animals preparation of bacteria suspension and then were respectively per- formed RGICA, RIHA and bacteria inspection. Results RGICA, RIHA and bacteria inspection were sensitive in 37 ℃ plague bacteria liquid and 37 ℃ plague bacteria infected animals prepared with bacteria suspension.RGICA, RIHA and bacteria inspection were not sensitive in 28 ℃ plague bacteria liquid and 28 ℃ plague bacteria infected animal prepared with bacteria suspension. Conclusion Because of its simple, rapid and sensitive characteristics, RIHA has been used as a diagnostic criteria for plague.the sensitivity of 28 ℃ colloidal gold paper is poor and the absence of fl-negative strains antigen detection may lead to misdiagnosis, it can only be used as a auxiliary diagnosis.