原癌基因c—myb与抑癌基因P27在子宫内膜癌中的表达及意义

Expression and clinical significance of proto-oncogene c-myb and tumor suppression gene P27 in endometrial carcinoma

目的探讨原癌基因c—myb与抑癌基因P27在子宫内膜癌中的表达及意义。方法采用免疫组化染色SP法对85例子宫内膜癌和63例同期因子宫肌瘤行子宫切除的正常子宫内膜组织中c-myb、P27蛋白进行检测，结合临床病理指标进行分析。结果85例子宫内膜癌组织中c—myb的阳性率74．12％明显高于63例正常子宫内膜组织15．84％（x^2=49．108，P〈0．001）；而子宫内膜癌组织中P27的阳性率47．06％明显低于正常子宫内膜组织88．89％（x^2=27．779，P〈0．001）。在临床分级Ⅰ-Ⅱ期和Ⅲ．Ⅳ期表达的阳性率中，c—myb分别为64．71％和88．24％，差异有统计学意义（x^2=5．887，P=0．015）；P27分别为52．94％和38．24％，差异无统计学意义（x^2=1．770，P=0．183）。在组织学分级中，c-myb在分化程度越低的子宫内膜癌组织中表达越高，差异有统计学意义（x^2=6．569，P=0．037）；P27在分化程度越低的子宫内膜癌组织中的表达越低，差异也有统计学意义（x^2=8．178，P=0．017）．c-myb、P27表达在肌层浸润深度及有无淋巴结转移间差异无统计学意义（P〉0．05）。结论c—myb参与子宫内膜癌发生、发展，P27抑制、阻滞子宫内膜癌细胞增生，两者可能作为子宫内膜癌基因治疗的靶位点，为子宫内膜癌的治疗提供新的思路。

Objective To analyze the expression and clinical significance of oncogene c-myb and tumor suppression gene P27 in endometrial carcinoma. Methods Immunohistochemical staining were used to detect the expression of c-myb and P27 in 85 endometrial carcinoma specimens and 63 normal endometrium specimens taken from patients with hysterectomy of uterine fibroids. We explored the relationship between there expression and clinical pathological features of endometrial carcinoma. Results The positive rates of c-myb ex- pression in endometrial carcinoma （74. 12% ） was significantly higher than that in normal endometrium （ 15.84 % ） ,x^2= 49.108, P 〈 0.001 ; The positive rates of P27 expression in endometrial carcinoma （47.06 % ） was significantly lower than that in normal endometrium （88.89%） ,x^2 = 27. 779., P 〈 0.001. The positive rates of c-myb expression in stage HI and IV group （88.24%）, was significantly higher than that in stage I and II group（64.71% ）, x^2= 5. 887,P = 0. 015; The positive rates of p27 expression in stage III and 1V group （52. 94% ）, in stage I and 11 group （38. 24% ）. There was no difference between them,x^2 = 1. 770, P = 0. 183 ;C-myb expression was gradually enhanced from highly differentiated to low differentiated endometrial cancer. There was a significant difference among these groups （x^2 = 6. 569, P = 0. 037 ） ; P27 expression was gradually decreased from highly differentiated to low differentiated endometrial cancer. There was a significant difference among these groups（x^2 = 8. 178 ,P =0. 017）. There was no significant difference between c-myb and P27 expression in the myometrium invasion and lymph node metastasis （P 〉 0.05 ）. Conclusion c-myb and P27 may participate in the occurrence and development of endometrial carcinoma. These two proteins might be used as a new tumor marker for diagnosis and prognosis evaluation of endometrial carcinoma.